XB-ART-59277Am J Physiol Cell Physiol October 1, 2022; 323 (4): C990-C1002.
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Corneal dystrophy mutations R125H and R804H disable SLC4A11 by altering the extracellular pH dependence of the intracellular pK that governs H+(OH-) transport.
Mutations in the H+(OH-) conductor SLC4A11 result in corneal endothelial dystrophy. In previous studies using mouse Slc4a11, we showed that the pK value that governs the intracellular pH-dependence of SLC4A11 (pKi) is influenced by pHe. We also showed that some mutations result in acidic or alkaline shifts in pKi, indicating that the pH-dependence of SLC4A11 is important for physiological function. An R125H mutant, located in the cytosolic amino-terminus of SLC4A11, apparently causes a complete loss of function, yet the anion transport inhibitor DIDS can partially rescue SLC4A11/R125H activity. In the present study we set out to determine whether the effect of R125H is explained by an extreme shift in pKi. In Xenopus oocytes, we measured SLC4A11-mediated H+(OH-) conductance while monitoring pHi. We find that  the human corneal variant SLC4A11 has a more acidic pKi than mouse Slc4a11 likely due to the presence of an N-terminal appendage  pKi for human SLC4A11 is acid-shifted by raising pHe to 10.00, and  R125H and R804H mutants mediate substantial H+(OH-) conductances at pHe=10.00 with pKi shifted into the wild-type range. These data suggest that the defect in each is a shift in pKi at physiological pHe, brought about by a disconnection in the mechanisms by which pHe influences pKi. Using de novo modeling, we show that R125 is located at the cytosolic dimer interface, and suggest that this interface is critical for relaying the influence of pHe on the external face of the transmembrane domain to the intracellular, pKi-determining regions.
PubMed ID: 35993514
Article link: Am J Physiol Cell Physiol
Genes referenced: slc4a11
References [+] :
Alka, Molecular phenotype of SLC4A11 missense mutants: Setting the stage for personalized medicine in corneal dystrophies. 2019, Pubmed