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Negretti MI
,
Böse N
,
Petri N
,
Kremnyov S
,
Tsikolia N
.
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Development of visceral left-right asymmetry in bilateria is based on initial symmetry breaking followed by subsequent asymmetric molecular patterning. An important step is the left-sided expression of transcription factor pitx2 which is mediated by asymmetric expression of the nodal morphogen in the leftlateral plate mesoderm of vertebrates. Processes leading to emergence of the asymmetric nodal domain differ depending on the mode of symmetry breaking. In Xenopus laevis and mouse embryos, the leftward fluid flow on the ventral surface of the left-rightorganizer leads through intermediate steps to enhanced activity of the nodal protein on the left side of the organizer and subsequent asymmetric nodal induction in the lateral plate mesoderm. In the chick embryo, asymmetric morphogenesis of axial organs leads to paraxial nodal asymmetry during the late gastrulation stage. Although it was shown that hedgehog signaling is required for initiation of the nodal expression, the mechanism of its asymmetry remains to be clarified. In this study, we established the activation of hedgehog signaling in early chick embryos to further study its role in the initiation of asymmetric nodal expression. Our data reveal that hedgehog signaling is sufficient to induce the nodal expression in competent domains of the chick embryo, while treatment of Xenopus embryos led to moderate nodal inhibition. We discuss the role of symmetry breaking and competence in the initiation of asymmetric gene expression.
FIGURE 3. Inhibition of nodal1 expression in the lateral plate mesoderm of X. laevis embryos after treatment with an activator of hedgehog signaling. (A) Treatment with SAG leads to inhibition of nodal1 expression as compared to control embryos. (B) Example of left-sided nodal1 expression; (C) example of suppressed nodal1 expression. Intersecting arrows indicate anatomical axes. Numbers at the base of columns represent a number of analyzed embryos. *p < 0.05 (0.023) as compared to control and two-proportions z-test.
Afzelius,
Prevalence and genetics of immotile-cilia syndrome and left-handedness.
2006, Pubmed
Afzelius,
Prevalence and genetics of immotile-cilia syndrome and left-handedness.
2006,
Pubmed
Blum,
Xenopus, an ideal model system to study vertebrate left-right asymmetry.
2009,
Pubmed
,
Xenbase
Blum,
Animal left-right asymmetry.
2018,
Pubmed
,
Xenbase
Bragina,
Smoothened agonist augments proliferation and survival of neural cells.
2010,
Pubmed
Campione,
The homeobox gene Pitx2: mediator of asymmetric left-right signaling in vertebrate heart and gut looping.
1999,
Pubmed
,
Xenbase
Cohen,
Ptch1 and Gli regulate Shh signalling dynamics via multiple mechanisms.
2015,
Pubmed
Cui,
Rotation of organizer tissue contributes to left-right asymmetry.
2009,
Pubmed
Danilchick,
Xenopus laevis: Practical uses in cell and molecular biology. Pictorial collage of embryonic stages.
1991,
Pubmed
,
Xenbase
Echelard,
Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity.
1993,
Pubmed
Essner,
Kupffer's vesicle is a ciliated organ of asymmetry in the zebrafish embryo that initiates left-right development of the brain, heart and gut.
2005,
Pubmed
Grande,
Nodal signalling is involved in left-right asymmetry in snails.
2009,
Pubmed
Gros,
Cell movements at Hensen's node establish left/right asymmetric gene expression in the chick.
2009,
Pubmed
,
Xenbase
Gurdon,
Embryonic induction--molecular prospects.
1987,
Pubmed
HAMBURGER,
A series of normal stages in the development of the chick embryo.
1951,
Pubmed
Hamada,
Diversity of left-right symmetry breaking strategy in animals.
2020,
Pubmed
,
Xenbase
Harland,
In situ hybridization: an improved whole-mount method for Xenopus embryos.
1991,
Pubmed
,
Xenbase
Hu,
Hedgehog participates in the establishment of left-right asymmetry during amphioxus development by controlling Cerberus expression.
2017,
Pubmed
Iulianella,
Ventral neural patterning in the absence of a Shh activity gradient from the floorplate.
2018,
Pubmed
Johnson,
Ectopic expression of Sonic hedgehog alters dorsal-ventral patterning of somites.
1994,
Pubmed
Kajikawa,
Nodal paralogues underlie distinct mechanisms for visceral left-right asymmetry in reptiles and mammals.
2020,
Pubmed
Kremnyov,
Divergent axial morphogenesis and early shh expression in vertebrate prospective floor plate.
2018,
Pubmed
,
Xenbase
Lebreton,
Molecular to organismal chirality is induced by the conserved myosin 1D.
2018,
Pubmed
Levin,
A molecular pathway determining left-right asymmetry in chick embryogenesis.
1995,
Pubmed
Levin,
Left/right patterning signals and the independent regulation of different aspects of situs in the chick embryo.
1997,
Pubmed
Lewis,
Reagents for developmental regulation of Hedgehog signaling.
2014,
Pubmed
,
Xenbase
Lobikin,
Early, nonciliary role for microtubule proteins in left-right patterning is conserved across kingdoms.
2012,
Pubmed
,
Xenbase
Logan,
The transcription factor Pitx2 mediates situs-specific morphogenesis in response to left-right asymmetric signals.
1998,
Pubmed
Lombardo,
Morphogenetic control of zebrafish cardiac looping by Bmp signaling.
2019,
Pubmed
Maerker,
Bicc1 and Dicer regulate left-right patterning through post-transcriptional control of the Nodal inhibitor Dand5.
2021,
Pubmed
,
Xenbase
Matsui,
Left-right asymmetry in zebrafish.
2012,
Pubmed
Meinhardt,
3D reconstitution of the patterned neural tube from embryonic stem cells.
2014,
Pubmed
Motoyama,
Differential requirement for Gli2 and Gli3 in ventral neural cell fate specification.
2003,
Pubmed
Männer,
Does an equivalent of the "ventral node" exist in chick embryos? A scanning electron microscopic study.
2001,
Pubmed
Männer,
The anatomy of cardiac looping: a step towards the understanding of the morphogenesis of several forms of congenital cardiac malformations.
2009,
Pubmed
,
Xenbase
Narita,
Sonic hedgehog expression in developing chicken digestive organs is regulated by epithelial-mesenchymal interactions.
1998,
Pubmed
Nonaka,
De novo formation of left-right asymmetry by posterior tilt of nodal cilia.
2005,
Pubmed
Onjiko,
Single-cell mass spectrometry with multi-solvent extraction identifies metabolic differences between left and right blastomeres in the 8-cell frog (Xenopus) embryo.
2016,
Pubmed
,
Xenbase
Otto,
Early left-right asymmetries during axial morphogenesis in the chick embryo.
2014,
Pubmed
Pagán-Westphal,
The transfer of left-right positional information during chick embryogenesis.
1998,
Pubmed
Patel,
Nodal signalling and the roles of the transcription factors SnR and Pitx2 in vertebrate left-right asymmetry.
1999,
Pubmed
Pieper,
Matrix-filled microcavities in the emerging avian left-right organizer.
2020,
Pubmed
Rebagliati,
Zebrafish nodal-related genes are implicated in axial patterning and establishing left-right asymmetry.
1998,
Pubmed
,
Xenbase
Ribes,
Distinct Sonic Hedgehog signaling dynamics specify floor plate and ventral neuronal progenitors in the vertebrate neural tube.
2010,
Pubmed
Roelink,
Floor plate and motor neuron induction by vhh-1, a vertebrate homolog of hedgehog expressed by the notochord.
1994,
Pubmed
,
Xenbase
Schröder,
Paraxial Nodal Expression Reveals a Novel Conserved Structure of the Left-Right Organizer in Four Mammalian Species.
2016,
Pubmed
Schweickert,
The nodal inhibitor Coco is a critical target of leftward flow in Xenopus.
2010,
Pubmed
,
Xenbase
Schweickert,
Cilia-driven leftward flow determines laterality in Xenopus.
2007,
Pubmed
,
Xenbase
Stephen,
The chicken left right organizer has nonmotile cilia which are lost in a stage-dependent manner in the talpid(3) ciliopathy.
2014,
Pubmed
,
Xenbase
Sydow,
An Early Chick Embryo Culture Device for Extended Continuous Observation.
2017,
Pubmed
Szenker-Ravi,
Discovery of a genetic module essential for assigning left-right asymmetry in humans and ancestral vertebrates.
2022,
Pubmed
,
Xenbase
Tisler,
Cilia are required for asymmetric nodal induction in the sea urchin embryo.
2016,
Pubmed
Todd,
Hedgehog signaling stimulates the formation of proliferating Müller glia-derived progenitor cells in the chick retina.
2015,
Pubmed
Tsiairis,
An Hh-dependent pathway in lateral plate mesoderm enables the generation of left/right asymmetry.
2009,
Pubmed
Tsikolia,
Paraxial left-sided nodal expression and the start of left-right patterning in the early chick embryo.
2012,
Pubmed
Tsikolia,
The role and limits of a gradient based explanation of morphogenesis: a theoretical consideration.
2006,
Pubmed
Tsukui,
Multiple left-right asymmetry defects in Shh(-/-) mutant mice unveil a convergence of the shh and retinoic acid pathways in the control of Lefty-1.
1999,
Pubmed
Vandenberg,
Serotonin has early, cilia-independent roles in Xenopus left-right patterning.
2013,
Pubmed
,
Xenbase
Warner,
Contribution of hedgehog signaling to the establishment of left-right asymmetry in the sea urchin.
2016,
Pubmed
Weisheit,
An efficient in situ hybridization protocol for multiple tissue sections and probes on miniaturized slides.
2002,
Pubmed
Zhu,
Cilia-driven asymmetric Hedgehog signalling determines the amphioxus left-right axis by controlling Dand5 expression.
2020,
Pubmed
