Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
J Cell Biol 2002 Nov 25;1594:541-7. doi: 10.1083/jcb.200207090.
Show Gene links Show Anatomy links

The Xenopus Xmus101 protein is required for the recruitment of Cdc45 to origins of DNA replication.

Van Hatten RA , Tutter AV , Holway AH , Khederian AM , Walter JC , Michael WM .

The initiation of eukaryotic DNA replication involves origin recruitment and activation of the MCM2-7 complex, the putative replicative helicase. Mini-chromosome maintenance (MCM)2-7 recruitment to origins in G1 requires origin recognition complex (ORC), Cdt1, and Cdc6, and activation at G1/S requires MCM10 and the protein kinases Cdc7 and S-Cdk, which together recruit Cdc45, a putative MCM2-7 cofactor required for origin unwinding. Here, we show that the Xenopus BRCA1 COOH terminus repeat-containing Xmus101 protein is required for loading of Cdc45 onto the origin. Xmus101 chromatin association is dependent on ORC, and independent of S-Cdk and MCM2-7. These results define a new factor that is required for Cdc45 loading. Additionally, these findings indicate that the initiation complex assembly pathway bifurcates early, after ORC association with the origin, and that two parallel pathways, one controlled by MCM2-7, and the other by Xmus101, cooperate to load Cdc45 onto the origin.

PubMed ID: 12438414
PMC ID: PMC2173091
Article link: J Cell Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: arhgef5 brca1 cdc45 cdc6 cdc7 cdt1 gmnn mcm10 mcm2 mcm7 mmut orc2 rpa1 topbp1

Article Images: [+] show captions
References [+] :
Araki, Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint. 1996, Pubmed