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Proc Natl Acad Sci U S A
2002 Nov 26;9924:15782-7. doi: 10.1073/pnas.242611099.
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Kidney-specific chloride channel, OmClC-K, predominantly expressed in the diluting segment of freshwater-adapted tilapia kidney.
Miyazaki H
,
Kaneko T
,
Uchida S
,
Sasaki S
,
Takei Y
.
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The kidney plays an important role in osmoregulation in freshwater teleosts, which are exposed to the danger of osmotic loss of Na(+) and Cl(-). However, ion-transport mechanisms in the kidney are poorly understood, and ion transporters of the fish nephron have not been identified thus far. From Mozambique tilapia, Oreochromis mossambicus, we have cloned a chloride channel, which is a homologue of the mammalian kidney-specific chloride channel, ClC-K. The cDNA of the channel, named OmClC-K, encodes a protein whose amino acid sequence has high homology to Xenopus and mammalian ClC-K (Xenopus ClC-K, 41.8%; rat ClC-K2, 40.9%; rat ClC-K1, 40.1%). The mRNA of OmClC-K was expressed exclusively in the kidney, and the expression level of mRNA was increased more in freshwater-adapted fish than seawater-adapted fish. The immunohistochemical study using a specific antibody showed that OmClC-K-positive cells were specifically located in the distalnephron segments. Immunoelectron microscopy further showed that immunoreaction of OmClC-K was recognizable on the structure of basolateral membrane infoldings in the distal tubule cells. The localization of OmClC-K and its induction in hypoosmotic media suggest that OmClC-K is involved in Cl(-) reabsorption in the distal tubule of freshwater-adapted tilapia.
Adachi,
Two isoforms of a chloride channel predominantly expressed in thick ascending limb of Henle's loop and collecting ducts of rat kidney.
1994, Pubmed,
Xenbase
Adachi,
Two isoforms of a chloride channel predominantly expressed in thick ascending limb of Henle's loop and collecting ducts of rat kidney.
1994,
Pubmed
,
Xenbase
Bae,
Protein kinase A regulates chloride conductance in endocytic vesicles from proximal tubule.
1990,
Pubmed
Birkenhäger,
Mutation of BSND causes Bartter syndrome with sensorineural deafness and kidney failure.
2001,
Pubmed
Burg,
Function of the thick ascending limb of Henle's loop.
1973,
Pubmed
Duan,
Molecular identification of a volume-regulated chloride channel.
1997,
Pubmed
Estévez,
Barttin is a Cl- channel beta-subunit crucial for renal Cl- reabsorption and inner ear K+ secretion.
2001,
Pubmed
,
Xenbase
Fahlke,
Subunit stoichiometry of human muscle chloride channels.
1997,
Pubmed
,
Xenbase
Fisher,
Cloning and characterization of CLCN5, the human kidney chloride channel gene implicated in Dent disease (an X-linked hereditary nephrolithiasis).
1995,
Pubmed
Gamba,
Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney.
1994,
Pubmed
,
Xenbase
Ho,
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.
1993,
Pubmed
,
Xenbase
Jentsch,
Properties of voltage-gated chloride channels of the ClC gene family.
1995,
Pubmed
Kamunde,
Fine structure of the nephron in the euryhaline teleost, Oreochromis niloticus.
1994,
Pubmed
Kawasaki,
Cloning and expression of a protein kinase C-regulated chloride channel abundantly expressed in rat brain neuronal cells.
1994,
Pubmed
,
Xenbase
Kobayashi,
Intrarenal and cellular localization of CLC-K2 protein in the mouse kidney.
2001,
Pubmed
Lloyd,
Characterisation of renal chloride channel, CLCN5, mutations in hypercalciuric nephrolithiasis (kidney stones) disorders.
1997,
Pubmed
,
Xenbase
Logan,
A micropuncture study of kidney function in the river lamprey Lampetra fluviatilis adapted to sea water.
1980,
Pubmed
Lorenz,
Heteromultimeric CLC chloride channels with novel properties.
1996,
Pubmed
,
Xenbase
Middleton,
Purification, reconstitution, and subunit composition of a voltage-gated chloride channel from Torpedo electroplax.
1994,
Pubmed
Miyazaki,
Molecular cloning of CLC chloride channels in Oreochromis mossambicus and their functional complementation of yeast CLC gene mutant.
1999,
Pubmed
,
Xenbase
Mulberg,
Modulation of coated vesicle chloride channel activity and acidification by reversible protein kinase A-dependent phosphorylation.
1991,
Pubmed
Nishimura,
Sodium chloride and water transport in the renal distal tubule of the rainbow trout.
1983,
Pubmed
Payne,
Alternatively spliced isoforms of the putative renal Na-K-Cl cotransporter are differentially distributed within the rabbit kidney.
1994,
Pubmed
Perry,
The chloride cell: structure and function in the gills of freshwater fishes.
1997,
Pubmed
Piermarini,
Immunochemical analysis of the vacuolar proton-ATPase B-subunit in the gills of a euryhaline stingray (Dasyatis sabina): effects of salinity and relation to Na(+)/K(+)-ATPase.
2001,
Pubmed
Reeves,
Calcium-dependent chloride channels in endosomes from rabbit kidney cortex.
1994,
Pubmed
Rocha,
Sodium chloride and water transport in the medullary thick ascending limb of Henle. Evidence for active chloride transport.
1973,
Pubmed
Sakamoto,
Identification of a new outwardly rectifying Cl- channel that belongs to a subfamily of the ClC Cl- channels.
1996,
Pubmed
Singer,
A divergent CFTR homologue: highly regulated salt transport in the euryhaline teleost F. heteroclitus.
1998,
Pubmed
,
Xenbase
Steinmeyer,
Primary structure and functional expression of a developmentally regulated skeletal muscle chloride channel.
1991,
Pubmed
,
Xenbase
Suzuki,
Identification by differential display of a hypertonicity-inducible inward rectifier potassium channel highly expressed in chloride cells.
1999,
Pubmed
Takeuchi,
Cloning, tissue distribution, and intrarenal localization of ClC chloride channels in human kidney.
1995,
Pubmed
Thiemann,
A chloride channel widely expressed in epithelial and non-epithelial cells.
1992,
Pubmed
,
Xenbase
Ura,
Localization of Na+, K(+)-ATPase in tissues of rabbit and teleosts using an antiserum directed against a partial sequence of the alpha-subunit.
1996,
Pubmed
Wilson,
NaCl uptake by the branchial epithelium in freshwater teleost fish: an immunological approach to ion-transport protein localization.
2000,
Pubmed
Yoshikawa,
Localization of rat CLC-K2 chloride channel mRNA in the kidney.
1999,
Pubmed
Zhou,
Primary structure and functional properties of an epithelial K channel.
1994,
Pubmed
,
Xenbase
van Slegtenhorst,
A gene from the Xp22.3 region shares homology with voltage-gated chloride channels.
1994,
Pubmed