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KCNE4 is an inhibitory subunit to the KCNQ1 channel.
Grunnet M
,
Jespersen T
,
Rasmussen HB
,
Ljungstrøm T
,
Jorgensen NK
,
Olesen SP
,
Klaerke DA
.
???displayArticle.abstract??? KCNE4 is a membrane protein belonging to a family of single transmembrane domain proteins known to have dramatic effect on the gating of certain potassium channels. However, no functional role of KCNE4 has been suggested so far. In the present paper we demonstrate that KCNE4 is an inhibitory subunit to KCNQ1 channels. Co-expression of KCNQ1 and KCNE4 in Xenopus oocytes completely inhibited the KCNQ1 current. This was reproduced in mammalian CHO-K1 cells. Experiments with delayed expression of mRNA coding for KCNE4 in KCNQ1-expressing oocytes suggested that KCNE4 exerts its effect on KCNQ1 channels already expressed in the plasma membrane. This notion was supported by immunocytochemical studies and Western blotting, showing no significant difference in plasma membrane expression of KCNQ1 channels in the presence or absence of KCNE4. The impact of KCNE4 on KCNQ1 was specific since no effect of KCNE4 could be detected if co-expressed with KCNQ2-5 channels or hERG1 channels. RT-PCR studies revealed high KCNE4 expression in embryos and adult uterus, where significant expression of KCNQ1 channels has also been demonstrated.
Abbott,
MiRP2 forms potassium channels in skeletal muscle with Kv3.4 and is associated with periodic paralysis.
2001, Pubmed,
Xenbase
Abbott,
MiRP2 forms potassium channels in skeletal muscle with Kv3.4 and is associated with periodic paralysis.
2001,
Pubmed
,
Xenbase
Abbott,
MiRP1 forms IKr potassium channels with HERG and is associated with cardiac arrhythmia.
1999,
Pubmed
,
Xenbase
Barhanin,
K(V)LQT1 and lsK (minK) proteins associate to form the I(Ks) cardiac potassium current.
1996,
Pubmed
,
Xenbase
Boucherot,
Regulation and properties of KCNQ1 (K(V)LQT1) and impact of the cystic fibrosis transmembrane conductance regulator.
2001,
Pubmed
,
Xenbase
Demolombe,
Differential expression of KvLQT1 and its regulator IsK in mouse epithelia.
2001,
Pubmed
Duggal,
Mutation of the gene for IsK associated with both Jervell and Lange-Nielsen and Romano-Ward forms of Long-QT syndrome.
1998,
Pubmed
Felipe,
Differential expression of Isk mRNAs in mouse tissue during development and pregnancy.
1994,
Pubmed
,
Xenbase
Fink,
A new K+ channel beta subunit to specifically enhance Kv2.2 (CDRK) expression.
1996,
Pubmed
,
Xenbase
Gould,
Imprinting of mouse Kvlqt1 is developmentally regulated.
1998,
Pubmed
Grunnet,
Apamin interacts with all subtypes of cloned small-conductance Ca2+-activated K+ channels.
2001,
Pubmed
,
Xenbase
Jentsch,
Neuronal KCNQ potassium channels: physiology and role in disease.
2000,
Pubmed
Jespersen,
Dual-function vector for protein expression in both mammalian cells and Xenopus laevis oocytes.
2002,
Pubmed
,
Xenbase
Kozak,
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
1987,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Kunzelmann,
Expression and function of colonic epithelial KvLQT1 K+ channels.
2001,
Pubmed
,
Xenbase
Lock,
Contribution of the IsK (MinK) potassium channel subunit to regulatory volume decrease in murine tracheal epithelial cells.
2000,
Pubmed
Mall,
Role of K(V)LQT1 in cyclic adenosine monophosphate-mediated Cl(-) secretion in human airway epithelia.
2000,
Pubmed
McDonald,
A minK-HERG complex regulates the cardiac potassium current I(Kr).
1997,
Pubmed
,
Xenbase
Melman,
Structural determinants of KvLQT1 control by the KCNE family of proteins.
2001,
Pubmed
Morgan,
Retroviral vectors containing putative internal ribosome entry sites: development of a polycistronic gene transfer system and applications to human gene therapy.
1992,
Pubmed
Neyroud,
A novel mutation in the potassium channel gene KVLQT1 causes the Jervell and Lange-Nielsen cardioauditory syndrome.
1997,
Pubmed
Piccini,
KCNE1-like gene is deleted in AMME contiguous gene syndrome: identification and characterization of the human and mouse homologs.
1999,
Pubmed
Priori,
Genetic and molecular basis of cardiac arrhythmias: impact on clinical management parts I and II.
1999,
Pubmed
Romey,
Molecular mechanism and functional significance of the MinK control of the KvLQT1 channel activity.
1997,
Pubmed
Sanguinetti,
Two components of cardiac delayed rectifier K+ current. Differential sensitivity to block by class III antiarrhythmic agents.
1990,
Pubmed
Sanguinetti,
Coassembly of K(V)LQT1 and minK (IsK) proteins to form cardiac I(Ks) potassium channel.
1996,
Pubmed
,
Xenbase
Schroeder,
A constitutively open potassium channel formed by KCNQ1 and KCNE3.
2000,
Pubmed
,
Xenbase
Shi,
Beta subunits promote K+ channel surface expression through effects early in biosynthesis.
1996,
Pubmed
Splawski,
Mutations in the hminK gene cause long QT syndrome and suppress IKs function.
1997,
Pubmed
,
Xenbase
Takumi,
Cloning of a membrane protein that induces a slow voltage-gated potassium current.
1988,
Pubmed
,
Xenbase
Tinel,
KCNE2 confers background current characteristics to the cardiac KCNQ1 potassium channel.
2000,
Pubmed
,
Xenbase
Tyson,
IsK and KvLQT1: mutation in either of the two subunits of the slow component of the delayed rectifier potassium channel can cause Jervell and Lange-Nielsen syndrome.
1997,
Pubmed
Unsöld,
KCNE1 reverses the response of the human K+ channel KCNQ1 to cytosolic pH changes and alters its pharmacology and sensitivity to temperature.
2000,
Pubmed
,
Xenbase
Wang,
Positional cloning of a novel potassium channel gene: KVLQT1 mutations cause cardiac arrhythmias.
1996,
Pubmed
Yu,
MinK-related peptide 1: A beta subunit for the HCN ion channel subunit family enhances expression and speeds activation.
2001,
Pubmed
,
Xenbase
Zhang,
minK-related peptide 1 associates with Kv4.2 and modulates its gating function: potential role as beta subunit of cardiac transient outward channel?
2001,
Pubmed
,
Xenbase
