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EMBO J
2006 Feb 22;254:834-45. doi: 10.1038/sj.emboj.7600953.
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Mammalian Emi2 mediates cytostatic arrest and transduces the signal for meiotic exit via Cdc20.
Shoji S
,
Yoshida N
,
Amanai M
,
Ohgishi M
,
Fukui T
,
Fujimoto S
,
Nakano Y
,
Kajikawa E
,
Perry AC
.
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Fertilizable mammalian oocytes are arrested at the second meiotic metaphase (mII) by the cyclinB-Cdc2 heterodimer, maturation promoting factor (MPF). MPF is stabilized via the activity of an unidentified cytostatic factor (CSF), thereby suspending meiotic progression until fertilization. We here present evidence that a conserved 71 kDa mammalian orthologue of Xenopus XErp1/Emi2, which we term endogenous meiotic inhibitor 2 (Emi2) is an essential CSF component. Depletion in situ of Emi2 by RNA interference elicited precocious meiotic exit in maturing mouse oocytes. Reduction of Emi2 released mature mII oocytes from cytostatic arrest, frequently inducing cytodegeneration. Mos levels autonomously declined to undetectable levels in mII oocytes. Recombinant Emi2 reduced the propensity of mII oocytes to exit meiosis in response to activating stimuli. Emi2 and Cdc20 proteins mutually interact and Cdc20 ablation negated the ability of Emi2 removal to induce metaphase release. Consistent with this, Cdc20 removal prevented parthenogenetic or sperm-induced meiotic exit. These studies show in intact oocytes that the interaction of Emi2 with Cdc20 links activating stimuli to meiotic resumption at fertilization and during parthenogenesis in mammals.
Alikani,
Cytoplasmic fragmentation in activated eggs occurs in the cytokinetic phase of the cell cycle, in lieu of normal cytokinesis, and in response to cytoskeletal disorder.
2005, Pubmed
Alikani,
Cytoplasmic fragmentation in activated eggs occurs in the cytokinetic phase of the cell cycle, in lieu of normal cytokinesis, and in response to cytoskeletal disorder.
2005,
Pubmed
Casaletto,
Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase.
2005,
Pubmed
,
Xenbase
Chang,
Degradation of APCcdc20 and APCcdh1 substrates during the second meiotic division in mouse eggs.
2004,
Pubmed
Charbonneau,
The onset of activation responsiveness during maturation coincides with the formation of the cortical endoplasmic reticulum in oocytes of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Choi,
The Mos/mitogen-activated protein kinase (MAPK) pathway regulates the size and degradation of the first polar body in maturing mouse oocytes.
1996,
Pubmed
,
Xenbase
Colledge,
Disruption of c-mos causes parthenogenetic development of unfertilized mouse eggs.
1994,
Pubmed
,
Xenbase
Dumont,
p90Rsk is not involved in cytostatic factor arrest in mouse oocytes.
2005,
Pubmed
,
Xenbase
Elbashir,
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.
2001,
Pubmed
Fang,
The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the anaphase-promoting complex to control anaphase initiation.
1998,
Pubmed
,
Xenbase
Fujimoto,
Mammalian phospholipase Czeta induces oocyte activation from the sperm perinuclear matrix.
2004,
Pubmed
Gross,
Induction of metaphase arrest in cleaving Xenopus embryos by the protein kinase p90Rsk.
1999,
Pubmed
,
Xenbase
Haccard,
Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase.
1993,
Pubmed
,
Xenbase
Haley,
Kinetic analysis of the RNAi enzyme complex.
2004,
Pubmed
Hannon,
Unlocking the potential of the human genome with RNA interference.
2004,
Pubmed
Hashimoto,
Parthenogenetic activation of oocytes in c-mos-deficient mice.
1994,
Pubmed
,
Xenbase
Huppi,
Defining and assaying RNAi in mammalian cells.
2005,
Pubmed
Jackson,
Expression profiling reveals off-target gene regulation by RNAi.
2003,
Pubmed
Kim,
Successful inactivation of endogenous Oct-3/4 and c-mos genes in mouse preimplantation embryos and oocytes using short interfering RNAs.
2002,
Pubmed
Kline,
Repetitive calcium transients and the role of calcium in exocytosis and cell cycle activation in the mouse egg.
1992,
Pubmed
Lefebvre,
Meiotic spindle stability depends on MAPK-interacting and spindle-stabilizing protein (MISS), a new MAPK substrate.
2002,
Pubmed
Li,
MAD2 associates with the cyclosome/anaphase-promoting complex and inhibits its activity.
1997,
Pubmed
,
Xenbase
Liu,
Calcium elevation at fertilization coordinates phosphorylation of XErp1/Emi2 by Plx1 and CaMK II to release metaphase arrest by cytostatic factor.
2005,
Pubmed
,
Xenbase
Lorca,
Calmodulin-dependent protein kinase II mediates inactivation of MPF and CSF upon fertilization of Xenopus eggs.
1993,
Pubmed
,
Xenbase
MARSTON,
THE FERTILIZABLE LIFE OF OVA AND THEIR MORPHOLOGY FOLLOWING DELAYED INSEMINATION IN MATURE AND IMMATURE MICE.
1964,
Pubmed
Margottin-Goguet,
Prophase destruction of Emi1 by the SCF(betaTrCP/Slimb) ubiquitin ligase activates the anaphase promoting complex to allow progression beyond prometaphase.
2003,
Pubmed
,
Xenbase
Markoulaki,
Fertilization stimulates long-lasting oscillations of CaMKII activity in mouse eggs.
2004,
Pubmed
Masui,
The elusive cytostatic factor in the animal egg.
2000,
Pubmed
Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed
Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase
Ohsumi,
Emi1-mediated M-phase arrest in Xenopus eggs is distinct from cytostatic factor arrest.
2004,
Pubmed
,
Xenbase
Okita,
Generation of optimized yellow and red fluorescent proteins with distinct subcellular localization.
2004,
Pubmed
Page,
The anaphase-promoting complex: new subunits and regulators.
1999,
Pubmed
Paronetto,
Functional interaction between p90Rsk2 and Emi1 contributes to the metaphase arrest of mouse oocytes.
2004,
Pubmed
Perry,
Hijacking oocyte DNA repair machinery in transgenesis?
2000,
Pubmed
Perry,
A novel trans-complementation assay suggests full mammalian oocyte activation is coordinately initiated by multiple, submembrane sperm components.
1999,
Pubmed
Pikó,
Amounts of mitochondrial DNA and abundance of some mitochondrial gene transcripts in early mouse embryos.
1987,
Pubmed
Rauh,
Calcium triggers exit from meiosis II by targeting the APC/C inhibitor XErp1 for degradation.
2005,
Pubmed
,
Xenbase
Reimann,
Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex.
2001,
Pubmed
,
Xenbase
Reimann,
Emi1 is required for cytostatic factor arrest in vertebrate eggs.
2002,
Pubmed
,
Xenbase
Runft,
Egg activation at fertilization: where it all begins.
2002,
Pubmed
Sagata,
The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs.
1989,
Pubmed
,
Xenbase
Saunders,
PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development.
2002,
Pubmed
Schmidt,
Xenopus polo-like kinase Plx1 regulates XErp1, a novel inhibitor of APC/C activity.
2005,
Pubmed
,
Xenbase
Smits,
Polo-like kinase-1 is a target of the DNA damage checkpoint.
2000,
Pubmed
Stokes,
DNA damage-induced replication arrest in Xenopus egg extracts.
2003,
Pubmed
,
Xenbase
Suzumori,
RFPL4 interacts with oocyte proteins of the ubiquitin-proteasome degradation pathway.
2003,
Pubmed
Svoboda,
Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference.
2000,
Pubmed
Tang,
Mad2-Independent inhibition of APCCdc20 by the mitotic checkpoint protein BubR1.
2001,
Pubmed
,
Xenbase
Terret,
DOC1R: a MAP kinase substrate that control microtubule organization of metaphase II mouse oocytes.
2003,
Pubmed
,
Xenbase
Terret,
The meiosis I-to-meiosis II transition in mouse oocytes requires separase activity.
2003,
Pubmed
Tong,
Polo-like kinase-1 is a pivotal regulator of microtubule assembly during mouse oocyte meiotic maturation, fertilization, and early embryonic mitosis.
2002,
Pubmed
Tsurumi,
The spindle assembly checkpoint is not essential for CSF arrest of mouse oocytes.
2004,
Pubmed
,
Xenbase
Tung,
A role for the anaphase-promoting complex inhibitor Emi2/XErp1, a homolog of early mitotic inhibitor 1, in cytostatic factor arrest of Xenopus eggs.
2005,
Pubmed
,
Xenbase
Tunquist,
The spindle checkpoint kinase bub1 and cyclin e/cdk2 both contribute to the establishment of meiotic metaphase arrest by cytostatic factor.
2002,
Pubmed
,
Xenbase
Tunquist,
Spindle checkpoint proteins Mad1 and Mad2 are required for cytostatic factor-mediated metaphase arrest.
2003,
Pubmed
,
Xenbase
Verlhac,
Mos is required for MAP kinase activation and is involved in microtubule organization during meiotic maturation in the mouse.
1996,
Pubmed
Watanabe,
Independent inactivation of MPF and cytostatic factor (Mos) upon fertilization of Xenopus eggs.
1991,
Pubmed
,
Xenbase
Wianny,
Specific interference with gene function by double-stranded RNA in early mouse development.
2000,
Pubmed
Yu,
Identification of a cullin homology region in a subunit of the anaphase-promoting complex.
1998,
Pubmed
Zernicka-Goetz,
Cytostatic factor inactivation is induced by a calcium-dependent mechanism present until the second cell cycle in fertilized but not in parthenogenetically activated mouse eggs.
1995,
Pubmed
