Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2002 Mar 01;215:1188-96. doi: 10.1093/emboj/21.5.1188.
Show Gene links
Show Anatomy links
Sequence-specific interaction of U1 snRNA with the SMN complex.
Yong J
,
Pellizzoni L
,
Dreyfuss G
.
???displayArticle.abstract???
The survival of motor neurons (SMN) protein complex functions in the biogenesis of spliceosomal small nuclear ribonucleoprotein particles (snRNPs) and prob ably other RNPs. All spliceosomal snRNPs have a common core of seven Sm proteins. To mediate the assembly of snRNPs, the SMN complex must be able to bring together Sm proteins with U snRNAs. We showed previously that SMN and other components of the SMN complex interact directly with several Sm proteins. Here, we show that the SMN complex also interacts specifically with U1 snRNA. The stem--loop 1 domain of U1 (SL1) is necessary and sufficient for SMN complex binding in vivo and in vitro. Substitution of three nucleotides in the SL1 loop (SL1A3) abolishes SMN interaction, and the corresponding U1 snRNA (U1A3) is impaired in U1 snRNP biogenesis. Microinjection of excess SL1 but not SL1A3 into Xenopus oocytes inhibits SMN complex binding to U1 snRNA and U1 snRNP assembly. These findings indicate that SMN complex interaction with SL1 is sequence-specific and critical for U1 snRNP biogenesis, further supporting the direct role of the SMN complex in RNP biogenesis.
Branlant,
The conformation of chicken, rat and human U1A RNAs in solution.
1981, Pubmed
Branlant,
The conformation of chicken, rat and human U1A RNAs in solution.
1981,
Pubmed
Branlant,
U2 RNA shares a structural domain with U1, U4, and U5 RNAs.
1982,
Pubmed
Burghes,
When is a deletion not a deletion? When it is converted.
1997,
Pubmed
Bühler,
Essential role for the tudor domain of SMN in spliceosomal U snRNP assembly: implications for spinal muscular atrophy.
1999,
Pubmed
,
Xenbase
Charroux,
Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli.
2000,
Pubmed
,
Xenbase
Charroux,
Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems.
1999,
Pubmed
Fischer,
An essential signaling role for the m3G cap in the transport of U1 snRNP to the nucleus.
1990,
Pubmed
,
Xenbase
Fischer,
Nucleo-cytoplasmic transport of U snRNPs: definition of a nuclear location signal in the Sm core domain that binds a transport receptor independently of the m3G cap.
1993,
Pubmed
,
Xenbase
Fischer,
The SMN-SIP1 complex has an essential role in spliceosomal snRNP biogenesis.
1997,
Pubmed
,
Xenbase
Friesen,
Specific sequences of the Sm and Sm-like (Lsm) proteins mediate their interaction with the spinal muscular atrophy disease gene product (SMN).
2000,
Pubmed
Friesen,
SMN, the product of the spinal muscular atrophy gene, binds preferentially to dimethylarginine-containing protein targets.
2001,
Pubmed
Friesen,
The methylosome, a 20S complex containing JBP1 and pICln, produces dimethylarginine-modified Sm proteins.
2001,
Pubmed
Gubitz,
Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins.
2002,
Pubmed
Hamm,
The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal.
1990,
Pubmed
,
Xenbase
Hamm,
In vitro assembly of U1 snRNPs.
1987,
Pubmed
,
Xenbase
Hamm,
Loop I of U1 small nuclear RNA is the only essential RNA sequence for binding of specific U1 small nuclear ribonucleoprotein particle proteins.
1988,
Pubmed
,
Xenbase
Hamm,
Multiple domains of U1 snRNA, including U1 specific protein binding sites, are required for splicing.
1990,
Pubmed
,
Xenbase
Hebert,
Coilin forms the bridge between Cajal bodies and SMN, the spinal muscular atrophy protein.
2001,
Pubmed
Imai,
A simple and rapid method for generating a deletion by PCR.
1991,
Pubmed
Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase
Jones,
Direct interaction of the spinal muscular atrophy disease protein SMN with the small nucleolar RNA-associated protein fibrillarin.
2001,
Pubmed
,
Xenbase
Kambach,
Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs.
1999,
Pubmed
Kataoka,
Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm.
2000,
Pubmed
,
Xenbase
Lefebvre,
Identification and characterization of a spinal muscular atrophy-determining gene.
1995,
Pubmed
Liu,
A novel nuclear structure containing the survival of motor neurons protein.
1996,
Pubmed
,
Xenbase
Liu,
Molecular characterization of the protein products of the fragile X syndrome gene and the survival of motor neurons gene.
1996,
Pubmed
Liu,
The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins.
1997,
Pubmed
Lorson,
The domain encoded by exon 2 of the survival motor neuron protein mediates nucleic acid binding.
1998,
Pubmed
Mattaj,
Nucleocytoplasmic transport and snRNP assembly.
1993,
Pubmed
Mattaj,
Ribonucleoprotein assembly: clues from spinal muscular atrophy.
1998,
Pubmed
,
Xenbase
Mattaj,
Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins.
1985,
Pubmed
,
Xenbase
Mattaj,
Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding.
1986,
Pubmed
,
Xenbase
Meister,
Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins.
2000,
Pubmed
Meister,
A multiprotein complex mediates the ATP-dependent assembly of spliceosomal U snRNPs.
2001,
Pubmed
,
Xenbase
Melki,
Spinal muscular atrophy.
1997,
Pubmed
Mount,
Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing.
1981,
Pubmed
Mourelatos,
SMN interacts with a novel family of hnRNP and spliceosomal proteins.
2001,
Pubmed
Nagai,
Structure and assembly of the spliceosomal snRNPs. Novartis Medal Lecture.
2001,
Pubmed
Nelissen,
The association of the U1-specific 70K and C proteins with U1 snRNPs is mediated in part by common U snRNP proteins.
1994,
Pubmed
Pellizzoni,
A functional interaction between the survival motor neuron complex and RNA polymerase II.
2001,
Pubmed
Pellizzoni,
A novel function for SMN, the spinal muscular atrophy disease gene product, in pre-mRNA splicing.
1998,
Pubmed
Pellizzoni,
SMN mutants of spinal muscular atrophy patients are defective in binding to snRNP proteins.
1999,
Pubmed
Pellizzoni,
The survival of motor neurons (SMN) protein interacts with the snoRNP proteins fibrillarin and GAR1.
2001,
Pubmed
Pellizzoni,
Purification of native survival of motor neurons complexes and identification of Gemin6 as a novel component.
2002,
Pubmed
Plessel,
m3G cap hypermethylation of U1 small nuclear ribonucleoprotein (snRNP) in vitro: evidence that the U1 small nuclear RNA-(guanosine-N2)-methyltransferase is a non-snRNP cytoplasmic protein that requires a binding site on the Sm core domain.
1994,
Pubmed
,
Xenbase
Query,
A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein.
1989,
Pubmed
Query,
A specific 31-nucleotide domain of U1 RNA directly interacts with the 70K small nuclear ribonucleoprotein component.
1989,
Pubmed
Scherly,
Identification of the RNA binding segment of human U1 A protein and definition of its binding site on U1 snRNA.
1989,
Pubmed
,
Xenbase
Stark,
Arrangement of RNA and proteins in the spliceosomal U1 small nuclear ribonucleoprotein particle.
2001,
Pubmed
Surowy,
Direct, sequence-specific binding of the human U1-70K ribonucleoprotein antigen protein to loop I of U1 small nuclear RNA.
1989,
Pubmed
Will,
Spliceosomal UsnRNP biogenesis, structure and function.
2001,
Pubmed
