Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-822
Genes Dev 2006 Jan 15;202:199-209. doi: 10.1101/gad.1383106.
Show Gene links Show Anatomy links

Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation.

Padmanabhan K , Richter JD .


???displayArticle.abstract???
CPEB is a sequence-specific RNA-binding protein that controls the polyadenylation-induced translation of mos and cyclin B1 mRNAs in maturing Xenopus oocytes. CPEB activity requires not only the phosphorylation of S174, but also the synthesis of a heretofore-unknown upstream effector molecule. We show that the synthesis of RINGO/Spy, an atypical activator of cyclin-dependent kinases (cdks), is necessary for CPEB-directed polyadenylation. Deletion analysis and mRNA reporter assays show that a cis element in the RINGO/Spy 3'UTR is necessary for translational repression in immature (G2-arrested) oocytes. The repression is mediated by 3'UTR Pumilio-Binding Elements (PBEs), and by its binding protein Pumilio 2 (Pum2). Pum2 also interacts with the Xenopus homolog of human Deleted for Azoospermia-like (DAZL) and the embryonic poly(A)-binding protein (ePAB). Following the induction of maturation, Pum2 dissociates not only from RINGO/Spy mRNA, but from XDAZL and ePAB as well; as a consequence, RINGO/Spy mRNA is translated. These results demonstrate that a reversible Pum2 interaction controls RINGO/Spy mRNA translation and, as a result, CPEB-mediated cytoplasmic polyadenylation.

???displayArticle.pubmedLink??? 16418484
???displayArticle.pmcLink??? PMC1356111
???displayArticle.link??? Genes Dev
???displayArticle.grants??? [+]

Species referenced: Xenopus
Genes referenced: cpeb1 dazl mos pabpc1l pabpc4 pum1 pum2 spdya

References [+] :
Ballantyne, A dependent pathway of cytoplasmic polyadenylation reactions linked to cell cycle control by c-mos and CDK1 activation. 1997, Pubmed, Xenbase