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Control of microtubule dynamics by Stu2p is essential for spindle orientation and metaphase chromosome alignment in yeast.
Kosco KA
,
Pearson CG
,
Maddox PS
,
Wang PJ
,
Adams IR
,
Salmon ED
,
Bloom K
,
Huffaker TC
.
Abstract
Stu2p is a member of a conserved family of microtubule-binding proteins and an essential protein in yeast. Here, we report the first in vivo analysis of microtubule dynamics in cells lacking a member of this protein family. For these studies, we have used a conditional Stu2p depletion strain expressing alpha-tubulin fused to green fluorescent protein. Depletion of Stu2p leads to fewer and less dynamic cytoplasmic microtubules in both G1 and preanaphase cells. The reduction in cytoplasmic microtubule dynamics is due primarily to decreases in both the catastrophe and rescue frequencies and an increase in the fraction of time microtubules spend pausing. These changes have significant consequences for the cell because they impede the ability of cytoplasmic microtubules to orient the spindle. In addition, recovery of fluorescence after photobleaching indicates that kinetochore microtubules are no longer dynamic in the absence of Stu2p. This deficiency is correlated with a failure to properly align chromosomes at metaphase. Overall, we provide evidence that Stu2p promotes the dynamics of microtubule plus-ends in vivo and that these dynamics are critical for microtubule interactions with kinetochores and cortical sites in the cytoplasm.
Adames,
Microtubule interactions with the cell cortex causing nuclear movements in Saccharomyces cerevisiae.
2000, Pubmed
Adames,
Microtubule interactions with the cell cortex causing nuclear movements in Saccharomyces cerevisiae.
2000,
Pubmed
Adams,
Localization of core spindle pole body (SPB) components during SPB duplication in Saccharomyces cerevisiae.
1999,
Pubmed
Bloom,
It's a kar9ochore to capture microtubules.
2000,
Pubmed
Carminati,
Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex.
1997,
Pubmed
Cassimeris,
Accessory protein regulation of microtubule dynamics throughout the cell cycle.
1999,
Pubmed
,
Xenbase
Charrasse,
The TOGp protein is a new human microtubule-associated protein homologous to the Xenopus XMAP215.
1998,
Pubmed
,
Xenbase
Chen,
The N terminus of the centromere H3-like protein Cse4p performs an essential function distinct from that of the histone fold domain.
2000,
Pubmed
Chen,
The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly.
1998,
Pubmed
Cormack,
Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans.
1997,
Pubmed
Cullen,
mini spindles: A gene encoding a conserved microtubule-associated protein required for the integrity of the mitotic spindle in Drosophila.
1999,
Pubmed
,
Xenbase
Davis,
Purification and biochemical characterization of tubulin from the budding yeast Saccharomyces cerevisiae.
1993,
Pubmed
Desai,
Microtubule polymerization dynamics.
1998,
Pubmed
Goshima,
Establishing biorientation occurs with precocious separation of the sister kinetochores, but not the arms, in the early spindle of budding yeast.
2000,
Pubmed
Haase,
Flow cytometric analysis of DNA content in budding yeast.
1997,
Pubmed
He,
Transient sister chromatid separation and elastic deformation of chromosomes during mitosis in budding yeast.
2000,
Pubmed
Kilmartin,
Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.
1982,
Pubmed
Knop,
Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation.
1998,
Pubmed
Maddox,
The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae.
2000,
Pubmed
Matthews,
ZYG-9, a Caenorhabditis elegans protein required for microtubule organization and function, is a component of meiotic and mitotic spindle poles.
1998,
Pubmed
Meluh,
Cse4p is a component of the core centromere of Saccharomyces cerevisiae.
1998,
Pubmed
Miller,
Bim1p/Yeb1p mediates the Kar9p-dependent cortical attachment of cytoplasmic microtubules.
2000,
Pubmed
Moqtaderi,
TBP-associated factors are not generally required for transcriptional activation in yeast.
1996,
Pubmed
Muhlrad,
A rapid method for localized mutagenesis of yeast genes.
1992,
Pubmed
Nabeshima,
p93dis1, which is required for sister chromatid separation, is a novel microtubule and spindle pole body-associating protein phosphorylated at the Cdc2 target sites.
1995,
Pubmed
Pasqualone,
STU1, a suppressor of a beta-tubulin mutation, encodes a novel and essential component of the yeast mitotic spindle.
1995,
Pubmed
Pearson,
Budding yeast chromosome structure and dynamics during mitosis.
2001,
Pubmed
Pereira,
Centrosome-microtubule nucleation.
1997,
Pubmed
,
Xenbase
Rieder,
The checkpoint delaying anaphase in response to chromosome monoorientation is mediated by an inhibitory signal produced by unattached kinetochores.
1995,
Pubmed
Severin,
Stu2 promotes mitotic spindle elongation in anaphase.
2001,
Pubmed
Shaw,
Imaging green fluorescent protein fusion proteins in Saccharomyces cerevisiae.
1998,
Pubmed
Shaw,
Astral microtubule dynamics in yeast: a microtubule-based searching mechanism for spindle orientation and nuclear migration into the bud.
1997,
Pubmed
Sherman,
Getting started with yeast.
1991,
Pubmed
Sikorski,
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
1989,
Pubmed
Sorger,
Factors required for the binding of reassembled yeast kinetochores to microtubules in vitro.
1994,
Pubmed
Stearns,
Motoring to the finish: kinesin and dynein work together to orient the yeast mitotic spindle.
1997,
Pubmed
Straight,
Mitosis in living budding yeast: anaphase A but no metaphase plate.
1997,
Pubmed
Sullivan,
Astral microtubules are not required for anaphase B in Saccharomyces cerevisiae.
1992,
Pubmed
Tanaka,
Cohesin ensures bipolar attachment of microtubules to sister centromeres and resists their precocious separation.
2000,
Pubmed
Tirnauer,
Yeast Bim1p promotes the G1-specific dynamics of microtubules.
1999,
Pubmed
Toso,
Kinetic stabilization of microtubule dynamic instability in vitro by vinblastine.
1993,
Pubmed
Tournebize,
Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase
Vasquez,
XMAP from Xenopus eggs promotes rapid plus end assembly of microtubules and rapid microtubule polymer turnover.
1994,
Pubmed
,
Xenbase
Wang,
Stu2p: A microtubule-binding protein that is an essential component of the yeast spindle pole body.
1997,
Pubmed
Wiese,
Gamma-tubulin complexes and their interaction with microtubule-organizing centers.
1999,
Pubmed
Winey,
Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle.
1995,
Pubmed
