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Am J Pathol
2001 Jun 01;1586:2021-9. doi: 10.1016/s0002-9440(10)64674-3.
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Dimerization co-factor of hepatocyte nuclear factor 1/pterin-4alpha-carbinolamine dehydratase is necessary for pigmentation in Xenopus and overexpressed in primary human melanoma lesions.
von Strandmann EP
,
Senkel S
,
Ryffel G
,
Hengge UR
.
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Dimerization co-factor of hepatocyte nuclear factor 1 (HNF1)/pterin-4alpha-carbinolamine dehydratase (DCoH/PCD) is both a positive co-factor of the HNF1 homeobox transcription factors and thus involved in gene regulation as well as an enzyme catalyzing the regeneration of tetrahydrobiopterin. Dysfunction of DCoH/PCD is associated with the human disorders hyperphenylalaninemia and vitiligo. In Xenopus, overexpression of the protein during development induces ectopic pigmentation. In this study loss of function experiments using DCoH/PCD-specific antibodies demonstrated that the protein is also absolutely necessary for pigment cell formation in Xenopus. In normal human skin DCoH/PCD protein is weakly expressed in the basal layer of the epidermis that consists of keratinocytes and melanocytes. Whereas only 4 of 25 benign nevi reacted with DCoH/PCD-specific antibodies, high protein levels were detectable in melanoma cell lines and 13 of 15 primary malignant melanoma lesions. The comparison with the commonly used melanoma markers S100 and HMB45 demonstrated that DCoH/PCD has an overlapping but distinct expression pattern in melanoma lesions. In addition to human colon cancer, this is the second report about the overexpression of DCoH/PCD in human tumor cells indicating that the protein might be involved in cancerogenesis.
Adler,
7-substituted pterins in humans with suspected pterin-4a-carbinolamine dehydratase deficiency. Mechanism of formation via non-enzymatic transformation from 6-substituted pterins.
1992, Pubmed
Adler,
7-substituted pterins in humans with suspected pterin-4a-carbinolamine dehydratase deficiency. Mechanism of formation via non-enzymatic transformation from 6-substituted pterins.
1992,
Pubmed
Anastasiadis,
Tetrahydrobiopterin uptake into rat brain synaptosomes, cultured PC12 cells, and rat striatum.
1994,
Pubmed
Cereghini,
Liver-enriched transcription factors and hepatocyte differentiation.
1996,
Pubmed
Citron,
Identity of 4a-carbinolamine dehydratase, a component of the phenylalanine hydroxylation system, and DCoH, a transregulator of homeodomain proteins.
1992,
Pubmed
Citron,
Mutation in the 4a-carbinolamine dehydratase gene leads to mild hyperphenylalaninemia with defective cofactor metabolism.
1993,
Pubmed
Cordell,
Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes).
1984,
Pubmed
Depaepe,
Pterin-4alpha-carbinolamine dehydratase in rat brain. I. Patterns of co-localization with tyrosine hydroxylase.
2000,
Pubmed
Endrizzi,
Crystal structure of DCoH, a bifunctional, protein-binding transcriptional coactivator.
1995,
Pubmed
Eskinazi,
Overexpression of pterin-4a-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 in human colon cancer.
1999,
Pubmed
Ficner,
Crystallization and preliminary crystallographic studies of recombinant dimerization cofactor of transcription factor HNF1/pterin-4 alpha-carbinolamine dehydratase from liver.
1995,
Pubmed
Ficner,
Three-dimensional structure of the bifunctional protein PCD/DCoH, a cytoplasmic enzyme interacting with transcription factor HNF1.
1995,
Pubmed
Goding,
Mitf from neural crest to melanoma: signal transduction and transcription in the melanocyte lineage.
2000,
Pubmed
Holewa,
HNF4beta, a new gene of the HNF4 family with distinct activation and expression profiles in oogenesis and embryogenesis of Xenopus laevis.
1997,
Pubmed
,
Xenbase
Johnen,
Studies on the enzymatic and transcriptional activity of the dimerization cofactor for hepatocyte nuclear factor 1.
1997,
Pubmed
Kapur,
Anti-melanoma monoclonal antibody HMB45 identifies an oncofetal glycoconjugate associated with immature melanosomes.
1992,
Pubmed
King,
Microphthalmia transcription factor. A sensitive and specific melanocyte marker for MelanomaDiagnosis.
1999,
Pubmed
Lei,
Expression of 4alpha-carbinolamine dehydratase in human epidermal keratinocytes.
1997,
Pubmed
Lei,
Identification of hepatic nuclear factor 1 binding sites in the 5' flanking region of the human phenylalanine hydroxylase gene: implication of a dual function of phenylalanine hydroxylase stimulator in the phenylalanine hydroxylation system.
1998,
Pubmed
Lei,
Characterization of expression of the gene for human pterin carbinolamine dehydratase/dimerization cofactor of HNF1.
1999,
Pubmed
Lister,
nacre encodes a zebrafish microphthalmia-related protein that regulates neural-crest-derived pigment cell fate.
1999,
Pubmed
Mayor,
Induction of the prospective neural crest of Xenopus.
1995,
Pubmed
,
Xenbase
McNutt,
The S100 family of multipurpose calcium-binding proteins.
1998,
Pubmed
Mendel,
Characterization of a cofactor that regulates dimerization of a mammalian homeodomain protein.
1991,
Pubmed
Nastos,
The embryonic expression of the tissue-specific transcription factor HNF1alpha in Xenopus: rapid activation by HNF4 and delayed induction by mesoderm inducers.
1998,
Pubmed
,
Xenbase
Peng,
Xenopus laevis: Practical uses in cell and molecular biology. Solutions and protocols.
1991,
Pubmed
,
Xenbase
Pogge v Strandmann,
Ectopic pigmentation in Xenopus in response to DCoH/PCD, the cofactor of HNF1 transcription factor/pterin-4alpha-carbinolamine dehydratase.
2000,
Pubmed
,
Xenbase
Pogge yon Strandmann,
Developmental expression of the maternal protein XDCoH, the dimerization cofactor of the homeoprotein LFB1 (HNF1).
1995,
Pubmed
,
Xenbase
Rhee,
The bifunctional protein DCoH modulates interactions of the homeodomain transcription factor HNF1 with nucleic acids.
1997,
Pubmed
Résibois,
Immunohistochemical localisation of pterin-4alpha-carbinolamine dehydratase in rat peripheral organs.
1999,
Pubmed
Schallreuter,
Regulation of melanin biosynthesis in the human epidermis by tetrahydrobiopterin.
1994,
Pubmed
Schallreuter,
Defective tetrahydrobiopterin and catecholamine biosynthesis in the depigmentation disorder vitiligo.
1994,
Pubmed
Schott,
Control of cell-cycle-associated tetrahydrobiopterin synthesis in rat thymocytes.
1992,
Pubmed
Sourdive,
The bifunctional DCOH protein binds to HNF1 independently of its 4-alpha-carbinolamine dehydratase activity.
1997,
Pubmed
Stebbins-Boaz,
CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase
Strandmann,
The bifunctional protein DCoH/PCD, a transcription factor with a cytoplasmic enzymatic activity, is a maternal factor in the rat egg and expressed tissue specifically during embryogenesis.
1998,
Pubmed
Tachibana,
Ectopic expression of MITF, a gene for Waardenburg syndrome type 2, converts fibroblasts to cells with melanocyte characteristics.
1996,
Pubmed
Thöny,
Hyperphenylalaninemia with high levels of 7-biopterin is associated with mutations in the PCBD gene encoding the bifunctional protein pterin-4a-carbinolamine dehydratase and transcriptional coactivator (DCoH).
1998,
Pubmed
Thöny,
Mutations in the pterin-4alpha-carbinolamine dehydratase (PCBD) gene cause a benign form of hyperphenylalaninemia.
1998,
Pubmed
Thöny,
Tetrahydrobiopterin biosynthesis, regeneration and functions.
2000,
Pubmed
Watanabe,
FAST-1 is a key maternal effector of mesoderm inducers in the early Xenopus embryo.
1999,
Pubmed
,
Xenbase
Zuckerman,
In vitro selection and characterization of a bleomycin-resistant subline of B16 melanoma.
1986,
Pubmed
