Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
EMBO J 2001 Apr 17;208:2015-27. doi: 10.1093/emboj/20.8.2015.
Show Gene links Show Anatomy links

Dimerization of the largest subunit of chromatin assembly factor 1: importance in vitro and during Xenopus early development.

Quivy JP , Grandi P , Almouzni G .

To date, the in vivo importance of chromatin assembly factors during development in vertebrates is unknown. Chromatin assembly factor 1 (CAF-1) represents the best biochemically characterized factor promoting chromatin assembly during DNA replication or repair in human cell-free systems. Here, we identify a Xenopus homologue of the largest subunit of CAF-1 (p150). Novel dimerization properties are found conserved in both Xenopus and human p150. A region of 36 amino acids required for p150 dimerization was identified. Deletion of this domain abolishes the ability of p150 to promote chromatin assembly in vitro. A dominant-negative interference based on these dimerization properties occurs both in vitro and in vivo. In the embryo, nuclear organization was severely affected and cell cycle progression was impaired during the rapid early cleaving stages of Xenopus development. We propose that the rapid proliferation at early developmental stages necessitates the unique properties of an assembly factor that can ensure a tight coupling between DNA replication or repair and chromatin assembly.

PubMed ID: 11296234
PMC ID: PMC125230
Article link: EMBO J

Species referenced: Xenopus
Genes referenced: abl1

References [+] :
Adams, Chromatin assembly: biochemical identities and genetic redundancy. 1999, Pubmed