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Mol Cell Biol
2000 Dec 01;2023:8676-83. doi: 10.1128/MCB.20.23.8676-8683.2000.
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Inhibitor of the tissue-specific transcription factor HNF4, a potential regulator in early Xenopus development.
Peiler G
,
Böckmann B
,
Nakhei H
,
Ryffel GU
.
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Hepatocyte nuclear factor 4alpha (HNF4alpha) is an orphan receptor of the nuclear receptor superfamily and expressed in vertebrates as a tissue-specific transcription factor in liver, kidney, intestine, stomach, and pancreas. It also plays a crucial role in early embryonic development and has been identified as a maternal component in the Xenopus egg. We now report on an activity present in Xenopus embryos that inhibits the DNA binding of HNF4. This HNF4 inhibitor copurifies with a 25-kDa protein under nondenaturing conditions but can be separated from this protein by sodium dodecyl sulfate treatment. Protease treatment of the inhibitor results in a core fragment of about 5 kDa that retains full inhibitory activity. The activity of the HNF4 inhibitor can also be monitored in the absence of DNA, as it alters the mobility of the HNF4 protein in native polyacrylamide gels and the accessibility of antibodies. Comparing the activity of the HNF4 inhibitor with acyl coenzyme A's, recently proposed to be ligands of HNF4, we observe a more stringent specificity for the HNF4 inhibitor activity. Using deletion constructs of the HNF4 protein, we could show that the potential ligand-binding domain of HNF4 is not required, and thus the HNF4 inhibitor does not represent a classical ligand as defined for the nuclear receptor superfamily. Based on our previous finding that maternal HNF4 is abundantly present in Xenopus embryos but the target gene HNF1alpha is only marginally expressed, we propose that the HNF4 inhibitor functions in the embryo to restrict the activity of the maternal HNF4 proteins.
Bartkowski,
Developmental regulation and tissue distribution of the liver transcription factor LFB1 (HNF1) in Xenopus laevis.
1993, Pubmed,
Xenbase
Bartkowski,
Developmental regulation and tissue distribution of the liver transcription factor LFB1 (HNF1) in Xenopus laevis.
1993,
Pubmed
,
Xenbase
Chen,
Disruption of the HNF-4 gene, expressed in visceral endoderm, leads to cell death in embryonic ectoderm and impaired gastrulation of mouse embryos.
1994,
Pubmed
Cho,
BMPs, Smads and metalloproteases: extracellular and intracellular modes of negative regulation.
1998,
Pubmed
Dale,
BMP signalling in early Xenopus development.
1999,
Pubmed
,
Xenbase
Dignam,
Preparation of extracts from higher eukaryotes.
1990,
Pubmed
Duncan,
Murine gastrulation requires HNF-4 regulated gene expression in the visceral endoderm: tetraploid rescue of Hnf-4(-/-) embryos.
1997,
Pubmed
Duncan,
Expression of transcription factor HNF-4 in the extraembryonic endoderm, gut, and nephrogenic tissue of the developing mouse embryo: HNF-4 is a marker for primary endoderm in the implanting blastocyst.
1994,
Pubmed
Forman,
Androstane metabolites bind to and deactivate the nuclear receptor CAR-beta.
1998,
Pubmed
Gorman,
High efficiency DNA-mediated transformation of primate cells.
1983,
Pubmed
Green,
Critical structural elements and multitarget protein interactions of the transcriptional activator AF-1 of hepatocyte nuclear factor 4.
1998,
Pubmed
Hertz,
Fatty acyl-CoA thioesters are ligands of hepatic nuclear factor-4alpha.
1998,
Pubmed
Holewa,
Transcriptional hierarchy in Xenopus embryogenesis: HNF4 a maternal factor involved in the developmental activation of the gene encoding the tissue specific transcription factor HNF1 alpha (LFB1).
1996,
Pubmed
,
Xenbase
Holewa,
HNF4beta, a new gene of the HNF4 family with distinct activation and expression profiles in oogenesis and embryogenesis of Xenopus laevis.
1997,
Pubmed
,
Xenbase
Jiang,
Serine/threonine phosphorylation of orphan receptor hepatocyte nuclear factor 4.
1997,
Pubmed
Ktistaki,
Modulation of hepatic gene expression by hepatocyte nuclear factor 1.
1997,
Pubmed
Kugler,
BAP, a rat liver protein that activates transcription through a promoter element with similarity to the USF/MLTF binding site.
1990,
Pubmed
,
Xenbase
Kuo,
A transcriptional hierarchy involved in mammalian cell-type specification.
1992,
Pubmed
Lai,
Hepatocyte nuclear factor 3 alpha belongs to a gene family in mammals that is homologous to the Drosophila homeotic gene fork head.
1991,
Pubmed
Lausen,
Naturally occurring mutations in the human HNF4alpha gene impair the function of the transcription factor to a varying degree.
2000,
Pubmed
Lee,
The orphan nuclear receptor SHP inhibits hepatocyte nuclear factor 4 and retinoid X receptor transactivation: two mechanisms for repression.
2000,
Pubmed
Li,
Mammalian hepatocyte differentiation requires the transcription factor HNF-4alpha.
2000,
Pubmed
Liu,
Characterization of rat CD14 promoter and its regulation by transcription factors AP1 and Sp family proteins in hepatocytes.
2000,
Pubmed
Miquerol,
Expression of the L-type pyruvate kinase gene and the hepatocyte nuclear factor 4 transcription factor in exocrine and endocrine pancreas.
1994,
Pubmed
Miyazono,
Positive and negative regulation of TGF-beta signaling.
2000,
Pubmed
Nakhei,
An alternative splice variant of the tissue specific transcription factor HNF4alpha predominates in undifferentiated murine cell types.
1998,
Pubmed
Nastos,
The embryonic expression of the tissue-specific transcription factor HNF1alpha in Xenopus: rapid activation by HNF4 and delayed induction by mesoderm inducers.
1998,
Pubmed
,
Xenbase
Nuclear Receptors Nomenclature Committee,
A unified nomenclature system for the nuclear receptor superfamily.
1999,
Pubmed
Ryffel,
Distinct promoter elements mediate endodermal and mesodermal expression of the HNF1alpha promoter in transgenic Xenopus.
2000,
Pubmed
,
Xenbase
Schorpp,
Hepatocyte-specific promoter element HP1 of the Xenopus albumin gene interacts with transcriptional factors of mammalian hepatocytes.
1988,
Pubmed
,
Xenbase
Sel,
Human renal cell carcinogenesis is accompanied by a coordinate loss of the tissue specific transcription factors HNF4 alpha and HNF1 alpha.
1996,
Pubmed
Sladek,
Modulation of transcriptional activation and coactivator interaction by a splicing variation in the F domain of nuclear receptor hepatocyte nuclear factor 4alpha1.
1999,
Pubmed
Sladek,
Liver-enriched transcription factor HNF-4 is a novel member of the steroid hormone receptor superfamily.
1990,
Pubmed
Stumpf,
The DNA binding activity of the liver transcription factors LFB1 (HNF1) and HNF4 varies coordinately in rat hepatocellular carcinoma.
1995,
Pubmed
Tacchini,
Differential activation of some transcription factors during rat liver ischemia, reperfusion, and heat shock.
1999,
Pubmed
Taraviras,
Characterization of the mouse HNF-4 gene and its expression during mouse embryogenesis.
1994,
Pubmed
Tian,
Tissue-specific expression of the gene encoding hepatocyte nuclear factor 1 may involve hepatocyte nuclear factor 4.
1991,
Pubmed
Viollet,
Protein kinase A-dependent phosphorylation modulates DNA-binding activity of hepatocyte nuclear factor 4.
1997,
Pubmed
Wang,
SRC-1 and GRIP1 coactivate transcription with hepatocyte nuclear factor 4.
1998,
Pubmed
Yamagata,
Mutations in the hepatocyte nuclear factor-1alpha gene in maturity-onset diabetes of the young (MODY3).
1996,
Pubmed
Yamagata,
Mutations in the hepatocyte nuclear factor-4alpha gene in maturity-onset diabetes of the young (MODY1).
1996,
Pubmed
Zapp,
Elements and factors involved in tissue-specific and embryonic expression of the liver transcription factor LFB1 in Xenopus laevis.
1993,
Pubmed
,
Xenbase
Zhong,
The expression pattern of a Drosophila homolog to the mouse transcription factor HNF-4 suggests a determinative role in gut formation.
1993,
Pubmed
Zhong,
Tissue-specific regulation of mouse hepatocyte nuclear factor 4 expression.
1994,
Pubmed
von Strandmann,
Patterning the expression of a tissue-specific transcription factor in embryogenesis: HNF1 alpha gene activation during Xenopus development.
1997,
Pubmed
,
Xenbase
