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Summary Attributions Wiki
XB-gRNA-23659080
gRNA Name: lhx1 gRNA1
Synonyms:
Genomic Target: lhx1

Sequence: 5' GGAGAAATGCTTCTCCAGGG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 19/20 19/20 19/20
genomic

gRNA		 5' CCCTGGAGAAGCATTTCTCG 3' ||||||||||||||||||| 3' GGGACCTCTTCGTAAAGAGG 5' 5' CCCTGGAGAAGCATTTCTCG 3' ||||||||||||||||||| 3' GGGACCTCTTCGTAAAGAGG 5' 5' CCCTGGAGAAGCATTTCTCG 3' ||||||||||||||||||| 3' GGGACCTCTTCGTAAAGAGG 5'
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr2L:61067130..61067149 19/20 Sense target lhx1.L
chr2S:61240154..61240173 19/20 Sense target lhx1.S
chr2L:154422199..154422218 16/20 Sense off-target postn.L
chr2S:134357886..134357905 16/20 Sense off-target postn.S
chr5L:18851978..18851997 19/20 unknown
chr7S:7011720..7011739 18/20 unknown
chr3L:69785061..69785080 17/20 unknown
chr4L:83155168..83155187 16/20 unknown
chr9_10L:47608877..47608896 16/20 unknown
tropicalis 10.0 Chr2:75675485..75675504 19/20 1 Sense target lhx1
Chr1:146145738..146145757 18/20 2 Sense off-target lhx5
Chr8:142290862..142290881 16/20 3 Sense off-target adar
Chr8:125706597..125706616 18/20 4 Antisense off-target cadm3
Chr5:94409537..94409556 17/20 5 Sense off-target adgrb3
Chr8:125066760..125066779 16/20 6 unknown
Publications
First: Tissue-Specific Gene Inactivation in Xenopus laevis: Knockout of lhx1 in the Kidney with CRISPR/Cas9., Genetics 2018                          
Most recent:
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