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Summary Attributions Wiki
XB-gRNA-23659081
gRNA Name: lhx1 gRNA2
Synonyms:
Genomic Target: lhx1

Sequence: 5' GGTGTGCGGGCTGTGCCCAG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 19/20 19/20 19/20
genomic

gRNA		 5' CTGGGCACAGCCCGCACACT 3' ||||||||||||||||||| 3' GACCCGTGTCGGGCGTGTGG 5' 5' CTGGGCACAGCCCGCACACT 3' ||||||||||||||||||| 3' GACCCGTGTCGGGCGTGTGG 5' 5' CTGGGCACAGCCCGCACACT 3' ||||||||||||||||||| 3' GACCCGTGTCGGGCGTGTGG 5'
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr2L:61065026..61065045 19/20 Sense target lhx1.L
chr2S:61242418..61242437 19/20 Sense target lhx1.S
tropicalis 10.0 Chr2:75677966..75677985 19/20 1 Sense target lhx1
Chr8:49455010..49455029 18/20 2 Sense off-target tenm1
Chr7:132651110..132651129 16/20 3 Sense off-target fbxo42
Chr8:49455010..49455029 18/20 4 Antisense off-target LOC101731292
Publications
First: Tissue-Specific Gene Inactivation in Xenopus laevis: Knockout of lhx1 in the Kidney with CRISPR/Cas9., Genetics 2018                          
Most recent:
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