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Summary Attributions Wiki
XB-gRNA-23659082

gRNA Name: lhx1 gRNA3
Synonyms:
Genomic Target: lhx1

Sequence: 5' GGCTCCCTTATGTGTCGGGT 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 18/20 19/20 19/20
genomic

gRNA
5' ACCCGGCACATAAGGGAGCA 3' ||||| ||||||||||||| 3' TGGGCTGTGTATTCCCTCGG 5' 5' ACCCGACACATAAGGGAGCA 3' ||||||||||||||||||| 3' TGGGCTGTGTATTCCCTCGG 5' 5' ACCCGACACATAAGGGAGCA 3' ||||||||||||||||||| 3' TGGGCTGTGTATTCCCTCGG 5'
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr2L:61064244..61064263 19/20 Antisense target lhx1.L
chr2S:61243226..61243245 19/20 Antisense target lhx1.S
chr1S:29384315..29384334 18/20 Antisense off-target tmem165.S
chr8S:96119175..96119194 16/20 Antisense off-target arhgef2.S
chr1L:185343182..185343201 18/20 Sense off-target marveld2.L
tropicalis 10.0 Chr2:75678970..75678989 18/20 1 Antisense target lhx1
Publications
First: Tissue-Specific Gene Inactivation in Xenopus laevis: Knockout of lhx1 in the Kidney with CRISPR/Cas9., Genetics 2018                          
Most recent:
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