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XB-GRNA-23659095
gRNA Name:
adprhl1 gRNA9
Synonyms: -e7-1S
Genomic Target: adprhl1
PAM Sequence: TGG
Sequence: 5' GCAGGAGAAGGTGGTGCCAC 3'
Synonyms: -e7-1S
Genomic Target: adprhl1
PAM Sequence: TGG
Sequence: 5' GCAGGAGAAGGTGGTGCCAC 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 20/20 | 18/20 | 20/20 | ||||
genome
gRNA
|
5' GTGGCACCACCTTCTCCTGC 3' |||||||||||||||||||| 3' CACCGTGGTGGAAGAGGACG 5' |
5' GTAGCACCACCTTCTCCTGA 3' || |||||||||||||||| 3' CACCGTGGTGGAAGAGGACG 5' |
5' GTGGCACCACCTTCTCCTGC 3' |||||||||||||||||||| 3' CACCGTGGTGGAAGAGGACG 5' |
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Genomic Alignments  |
Position | Identity | Strand | Genomic Target | |||
| laevis 10.1 | Chr2S:106633795..106633814 | 20/20 | Sense | target | adprhl1.S | ||
| Chr2L:120477591..120477610 | 18/20 | Sense | target | adprhl1.L | |||
| Chr6L:52909862..52909881 | 18/20 | Antisense | off-target | ano10.L | |||
| Chr3L:144546902..144546921 | 18/20 | Sense | off-target | LOC108710362 | |||
| Chr4L:153176252..153176271 | 16/20 | Antisense | off-target | LOC121403137 | |||
| Chr4L:53225872..53225891 | 16/20 | Sense | off-target | LOC121402811 | |||
| Chr1S:85229113..85229132 | 18/20 | unknown | |||||
| Chr2S:41439204..41439223 | 18/20 | unknown | |||||
| Chr7S:9964825..9964844 | 18/20 | unknown | |||||
| Chr9_10L:20097171..20097190 | 17/20 | unknown | tropicalis 10.0 | Chr2:115908672..115908691 | 20/20 | Sense | adprhl1 |
| Chr1:60148150..60148169 | 18/20 | Antisense | il15 | ||||
| Chr3:33131291..33131310 | 18/20 | Antisense | slit3 | ||||
| Chr1:43711244..43711263 | 18/20 | Antisense | mtnr1a | ||||
| Chr7:37064660..37064679 | 18/20 | Antisense | hectd2 | ||||
| Chr6:57137940..57137959 | 17/20 | Sense | stk17a | ||||
| Chr8:141492514..141492533 | 17/20 | Sense | slc25a44 | ||||
| Chr3:9299611..9299630 | 16/20 | Sense | adipor2 | ||||
| Chr1:56450796..56450815 | 16/20 | Antisense | LOC116408478 | ||||
| Chr7:11982306..11982325 | 19/20 | ||||||
| Chr4:125119251..125119270 | 18/20 | ||||||
| Chr5:139197476..139197495 | 18/20 | ||||||
| Chr1:56198954..56198973 | 16/20 | ||||||
| Chr1:56221827..56221846 | 16/20 | ||||||
| Chr1:56336046..56336065 | 16/20 | ||||||
| Chr1:56386134..56386153 | 16/20 | ||||||
| Chr1:56467267..56467286 | 16/20 | ||||||
| Publications | |||||||
| ???morpholino.displaySummary.papersFirst??? |
Defective heart chamber growth and myofibrillogenesis after knockout of adprhl1 gene function by targeted disruption of the ancestral catalytic active site., PLoS One 2020  |
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| ???morpholino.displaySummary.papersRecent??? | |||||||
| ???morpholino.displaySummary.papersAll??? | |||||||
