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XB-gRNA-23659113
gRNA Name:
nuak1 gRNA1
Synonyms:
Genomic Target: nuak1
PAM Sequence: GGG
Sequence: 5' GAGACGAAGACTGAGTGAAA 3'
Synonyms:
Genomic Target: nuak1
PAM Sequence: GGG
Sequence: 5' GAGACGAAGACTGAGTGAAA 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 20/20 | 20/20 | 19/20 | ||||
| genomic gRNA |
5' TTTCACTCAGTCTTCGTCTC 3' |||||||||||||||||||| 3' AAAGTGAGTCAGAAGCAGAG 5' | 5' TTTCACTCAGTCTTCGTCTC 3' |||||||||||||||||||| 3' AAAGTGAGTCAGAAGCAGAG 5' | 5' TTTCGCTCAGTCTTCGTCTC 3' |||| ||||||||||||||| 3' AAAGTGAGTCAGAAGCAGAG 5' | ||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | ||
| laevis 9.2 | chr3L:44430076..44430095 | 20/20 | Sense | target | nuak1.L | ||
| chr3S:93815954..93815973 | 19/20 | Sense | target | nuak1.S | |||
| chr9_10L:104255752..104255771 | 17/20 | Antisense | off-target | ankfn1l.L | tropicalis 10.0 | Chr3:51686283..51686302 | 20/20 | 1 Sense | target | nuak1 |
| Chr9:88790358..88790377 | 16/20 | 2 Antisense | off-target | sema5b | |||
| Chr7:20659672..20659691 | 17/20 | 3 Sense | off-target | inpp5a.1 | |||
| Publications | |||||||
| First: |
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020  |
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| Most recent: | |||||||
| View All Papers | |||||||
