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XB-gRNA-23659126
gRNA Name:
kdm6a gRNA1
Synonyms:
Genomic Target: kdm6a
PAM Sequence: AGG
Sequence: 5' GGTAAAGGTGGATAGGGTGG 3'
Synonyms:
Genomic Target: kdm6a
PAM Sequence: AGG
Sequence: 5' GGTAAAGGTGGATAGGGTGG 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 20/20 | 19/20 | |||||
| genomic gRNA |
5' CCACCCTATCCACCTTTACC 3' |||||||||||||||||||| 3' GGTGGGATAGGTGGAAATGG 5' | 5' CCACCTTATCCACCTTTACC 3' ||||| |||||||||||||| 3' GGTGGGATAGGTGGAAATGG 5' | |||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | ||
| laevis 9.2 | chr2S:45003423..45003442 | 19/20 | Antisense | target | kdm6a.S | ||
| chr1S:115710217..115710236 | 18/20 | Sense | off-target | cdh24.S | |||
| chr7L:36323298..36323317 | 18/20 | Antisense | off-target | ascc1.L | |||
| chr9_10L:19817416..19817435 | 17/20 | Antisense | off-target | notum.L | |||
| chr8L:110983704..110983723 | 16/20 | Antisense | off-target | LOC108699404 | |||
| chr2L:77843198..77843217 | 20/20 | Antisense | off-target | LOC108708200 [provisional:kdm6a] | |||
| chr3S:112203984..112204003 | 17/20 | Antisense | off-target | LOC108712410 [provisional:slit3] | |||
| Scaffold85:418816..418835 | 17/20 | unknown | |||||
| Scaffold95:10062..10081 | 16/20 | unknown | |||||
| chr1S:78000569..78000588 | 16/20 | unknown | |||||
| chr2L:175909109..175909128 | 16/20 | unknown | |||||
| chr2S:49638690..49638709 | 16/20 | unknown | |||||
| chr3S:11153413..11153432 | 16/20 | unknown | |||||
| chr5L:69730620..69730639 | 16/20 | unknown | |||||
| chr6S:52298965..52298984 | 16/20 | unknown | |||||
| chr7L:48430945..48430964 | 16/20 | unknown | |||||
| chr8L:63800324..63800343 | 16/20 | unknown | tropicalis 10.0 | Chr2:55776368..55776387 | 20/20 | 1 Antisense | target | kdm6a |
| Chr10:21547192..21547211 | 16/20 | 2 Antisense | off-target | aoc3 | |||
| Chr8:34306789..34306808 | 17/20 | 3 Antisense | off-target | pappa | |||
| Chr7:64180942..64180961 | 16/20 | 4 Antisense | off-target | pex14 | |||
| Chr9:44800744..44800763 | 16/20 | 5 Antisense | off-target | pgap1 | |||
| Chr6:99186322..99186341 | 17/20 | 6 Antisense | off-target | znf516 | |||
| Chr6:73342466..73342485 | 16/20 | 7 Sense | off-target | tmem245 | |||
| Chr8:34306789..34306808 | 17/20 | 8 Sense | off-target | LOC116406671 | |||
| Chr1:115270873..115270892 | 18/20 | 9 | unknown | ||||
| Chr5:68117036..68117055 | 18/20 | 10 | unknown | ||||
| Chr2:75226187..75226206 | 16/20 | 11 | unknown | ||||
| Chr4:125037846..125037865 | 16/20 | 12 | unknown | ||||
| Chr7:32311710..32311729 | 16/20 | 13 | unknown | ||||
| Publications | |||||||
| First: |
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020  |
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| Most recent: | |||||||
| View All Papers | |||||||
