|
XB-gRNA-23659129
gRNA Name:
nf1 gRNA1
Synonyms: nf1_g1
Genomic Target: nf1
PAM Sequence: CGG
Sequence: 5' TGTAGAAATACGGCTGAAGA 3'
Synonyms: nf1_g1
Genomic Target: nf1
PAM Sequence: CGG
Sequence: 5' TGTAGAAATACGGCTGAAGA 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 20/20 | 19/20 | |||||
| genomic gRNA |
5' TCTTCAGCCGTATTTCTACA 3' |||||||||||||||||||| 3' AGAAGTCGGCATAAAGATGT 5' | 5' TCTTTAGCCGTATTTCTACA 3' |||| ||||||||||||||| 3' AGAAGTCGGCATAAAGATGT 5' | |||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | ||
| laevis 9.2 | chr2S:87149357..87149376 | 19/20 | Antisense | target | nf1.S | ||
| Scaffold20:3436881..3436900 | 18/20 | unknown | |||||
| chr1L:60524035..60524054 | 16/20 | unknown | tropicalis 10.0 | Chr2:104771087..104771106 | 20/20 | Antisense | target | nf1 |
| Chr4:134207300..134207319 | 17/20 | Antisense | off-target | plxna1 | |||
| Publications | |||||||
| First: |
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020  |
||||||
| Most recent: | |||||||
| View All Papers | |||||||
