|
XB-gRNA-23659130
gRNA Name:
nf1 gRNA2
Synonyms: nf1_g2
Genomic Target: nf1
PAM Sequence: CGG
Sequence: 5' CGAAGCAGACATTCGGAGTT 3'
Synonyms: nf1_g2
Genomic Target: nf1
PAM Sequence: CGG
Sequence: 5' CGAAGCAGACATTCGGAGTT 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 20/20 | 20/20 | 20/20 | ||||
| genomic gRNA |
5' AACTCCGAATGTCTGCTTCG 3' |||||||||||||||||||| 3' TTGAGGCTTACAGACGAAGC 5' | 5' AACTCCGAATGTCTGCTTCG 3' |||||||||||||||||||| 3' TTGAGGCTTACAGACGAAGC 5' | 5' AACTCCGAATGTCTGCTTCG 3' |||||||||||||||||||| 3' TTGAGGCTTACAGACGAAGC 5' | ||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | ||
| laevis 9.2 | chr2L:102874624..102874643 | 20/20 | Antisense | target | nf1.L | ||
| chr2S:87149417..87149436 | 20/20 | Antisense | target | nf1.S | |||
| chr4S:41727175..41727194 | 17/20 | Antisense | off-target | sntb2.S | |||
| chr1L:26998755..26998774 | 19/20 | unknown | |||||
| chr8S:13473851..13473870 | 18/20 | unknown | |||||
| chr7L:4037064..4037083 | 17/20 | unknown | tropicalis 10.0 | Chr2:104771147..104771166 | 20/20 | Antisense | target | nf1 |
| Publications | |||||||
| First: |
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020  |
||||||
| Most recent: | |||||||
| View All Papers | |||||||
