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Summary Attributions Wiki
XB-gRNA-23659131
gRNA Name: notch1 gRNA1
Synonyms:
Genomic Target: notch1

PAM Sequence: CGG
Sequence: 5' GACGGACCTGCAACAAATGT 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 19/20 19/20
genome
gRNA
 
5' ACATTTGTTGCAGGTCCGTC 3'
   ||||||||||||||||||||
3' TGTAAACAACGTCCAGGCAG 5'
 
5' ACATCTGTTGCAGGTCCGTC 3'
   |||| |||||||||||||||
3' TGTAAACAACGTCCAGGCAG 5'
 
5' ACATTTGCTGCAGGTCCGTC 3'
   ||||||| ||||||||||||
3' TGTAAACAACGTCCAGGCAG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr8L:4295102..4295121 19/20 Sense target notch1.L
Chr8S:29215879..29215898 19/20 Sense target notch1.S
Chr2L:152341302..152341321 19/20 Sense off-target endou.L
Chr7S:27908432..27908451 17/20 Sense off-target zmat4.S
Chr5S:137064671..137064690 17/20 unknown
Chr6L:19441982..19442001 17/20 unknown
Chr3S:122475296..122475315 16/20 unknown
Chr3S:76303343..76303362 16/20 unknown
Chr1S:6560285..6560304 16/20 unknown
tropicalis 10.0 Chr8:6561840..6561859 20/20 Sense target notch1
Chr5:7827170..7827189 17/20 Antisense off-target itpkb
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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