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Summary Attributions Wiki
XB-gRNA-23659161
gRNA Name: hmgcr gRNA1
Synonyms: hmgcr_L
Genomic Target: hmgcr

PAM Sequence: AGG
Sequence: 5' GCCCGTGGCATGGCAATTCT 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 19/20 20/20 20/20
genomic

gRNA		 5' AAAATTGCCATGCCACGGGC 3' | |||||||||||||||||| 3' TCTTAACGGTACGGTGCCCG 5' 5' AGAATTGCCATGCCACGGGC 3' |||||||||||||||||||| 3' TCTTAACGGTACGGTGCCCG 5' 5' AGAATTGCCATGCCACGGGC 3' |||||||||||||||||||| 3' TCTTAACGGTACGGTGCCCG 5'
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr1L:187447098..187447117 20/20 Sense target hmgcr.L
chr1S:156431941..156431960 20/20 Sense target hmgcr.S
chr7S:19313861..19313880 18/20 unknown
tropicalis 10.0 Chr1:184201686..184201705 19/20 1 Sense target hmgcr
Chr8:60585574..60585593 18/20 2 Sense off-target pcdh11x
Chr3:103996280..103996299 19/20 3 unknown
Publications
First: Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020                        
Most recent:
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