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XB-gRNA-23659356
gRNA Name:
dysf gRNA1
Synonyms: dysferlin, trop dysf T3
Genomic Target: dysf
PAM Sequence: AGG
Sequence: 5' CTTCTCCAAGACCAGAGAAGAGG 3'
Synonyms: dysferlin, trop dysf T3
Genomic Target: dysf
PAM Sequence: AGG
Sequence: 5' CTTCTCCAAGACCAGAGAAGAGG 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 23/23 | ||||||
| genomic gRNA |
5' CCTCTTCTCTGGTCTTGGAGAAG 3' ||||||||||||||||||||||| 3' GGAGAAGAGACCAGAACCTCTTC 5' | ||||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | ||
| laevis 10.1 | Chr4L:107421941..107421963 | 20/23 | Sense | off-target | vav3.L | ||
| Chr4S:90095742..90095764 | 20/23 | Sense | off-target | vav3.S | |||
| Chr7S:5967684..5967706 | 20/23 | Sense | off-target | prkg1.S | |||
| Chr2L:56775300..56775322 | 20/23 | Antisense | off-target | col8a2.L | |||
| Chr7L:8376103..8376125 | 21/23 | Sense | off-target | LOC108696071 | |||
| Chr9_10L:130244491..130244513 | 19/23 | Antisense | off-target | LOC100037197 | |||
| Chr8L:102964947..102964969 | 20/23 | Sense | off-target | arhgap11a.1.L | |||
| Chr6L:153959155..153959177 | 19/23 | unknown | |||||
| Chr6S:130468836..130468858 | 19/23 | unknown | tropicalis 10.0 | Chr7:38721213..38721235 | 23/23 | 1 Sense | target | dysf |
| Chr4:110709029..110709051 | 20/23 | 2 Sense | off-target | vav3 | |||
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