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XB-gRNA-23659364
gRNA Name: eya1 gRNA3
Synonyms: laevis eya1 T3
Genomic Target: eya1

PAM Sequence: GGG
Sequence: 5' GGACAGACACAATTCTCCTCGGG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 23/23 23/23 23/23
genomic

gRNA		 5' CCCGAGGAGAATTGTGTCTGTCC 3' ||||||||||||||||||||||| 3' GGGCTCCTCTTAACACAGACAGG 5' 5' CCCGAGGAGAATTGTGTCTGTCC 3' ||||||||||||||||||||||| 3' GGGCTCCTCTTAACACAGACAGG 5' 5' CCCGAGGAGAATTGTGTCTGTCC 3' ||||||||||||||||||||||| 3' GGGCTCCTCTTAACACAGACAGG 5'
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr6L:129135329..129135351 23/23 Sense target eya1.L
Chr6S:106175186..106175208 23/23 Sense target eya1.S
Chr1S:197013505..197013527 19/23 unknown
Chr1S:29279466..29279488 19/23 unknown
tropicalis 10.0 Chr6:120533948..120533970 23/23 1 Sense target eya1
Chr5:97925593..97925615 19/23 2 Antisense off-target csmd1
Chr1:188017063..188017085 19/23 3 Sense off-target adamts6
Chr2:109653226..109653248 19/23 4 unknown
Chr6:117077683..117077705 19/23 5 unknown
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