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XB-gRNA-23659383
gRNA Name:
hmgcr gRNA2
Synonyms: laevis hmgcr T5
Genomic Target: hmgcr
PAM Sequence: AGG
Sequence: 5' GCCCGTGGCATGGCAATTCTAGG 3'
Synonyms: laevis hmgcr T5
Genomic Target: hmgcr
PAM Sequence: AGG
Sequence: 5' GCCCGTGGCATGGCAATTCTAGG 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 22/23 | 23/23 | 23/23 | ||||
| genomic gRNA |
5' CCTAAAATTGCCATGCCACGGGC 3' |||| |||||||||||||||||| 3' GGATCTTAACGGTACGGTGCCCG 5' | 5' CCTAGAATTGCCATGCCACGGGC 3' ||||||||||||||||||||||| 3' GGATCTTAACGGTACGGTGCCCG 5' | 5' CCTAGAATTGCCATGCCACGGGC 3' ||||||||||||||||||||||| 3' GGATCTTAACGGTACGGTGCCCG 5' | ||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | ||
| laevis 9.2 | chr1L:187447098..187447120 | 23/23 | Sense | target | hmgcr.L | ||
| chr1S:156431941..156431963 | 23/23 | Sense | target | hmgcr.S | |||
| chr2S:82364280..82364302 | 19/23 | unknown | |||||
| chr5L:104543355..104543377 | 19/23 | unknown | tropicalis 10.0 | Chr1:184201686..184201708 | 22/23 | 1 Sense | target | hmgcr |
| Chr8:60585571..60585593 | 20/23 | 2 Sense | off-target | pcdh11x | |||
| Chr3:103996280..103996302 | 21/23 | 3 | unknown | ||||
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