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XB-gRNA-23659392
gRNA Name:
irx3 gRNA1
Synonyms: trop irx3 T2
Genomic Target: irx3
PAM Sequence: AGG
Sequence: 5' AAAGAACCCATATCCGACCAAGG 3'
Synonyms: trop irx3 T2
Genomic Target: irx3
PAM Sequence: AGG
Sequence: 5' AAAGAACCCATATCCGACCAAGG 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 23/23 | 22/23 | 22/23 | ||||
| genomic gRNA |
5' CCTTGGTCGGATATGGGTTCTTT 3' ||||||||||||||||||||||| 3' GGAACCAGCCTATACCCAAGAAA 5' | 5' CCTTGGTCGGATAGGGGTTCTTT 3' ||||||||||||| ||||||||| 3' GGAACCAGCCTATACCCAAGAAA 5' | 5' CCTTGGTCGGATACGGGTTCTTT 3' ||||||||||||| ||||||||| 3' GGAACCAGCCTATACCCAAGAAA 5' | ||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | ||
| laevis 9.2 | chr4L:31935291..31935313 | 22/23 | Sense | target | irx3.L | ||
| chr4S:24047035..24047057 | 22/23 | Sense | target | irx3.S | |||
| chr6L:66217957..66217979 | 21/23 | Sense | off-target | irx1.L | |||
| chr6S:53562930..53562952 | 21/23 | Sense | off-target | irx1.S | |||
| chr9_10S:1007397..1007419 | 20/23 | unknown | |||||
| chr1S:21390848..21390870 | 19/23 | unknown | tropicalis 10.0 | Chr4:41853106..41853128 | 23/23 | Sense | target | irx3 |
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