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Summary Attributions Wiki
XB-GRNA-23659397
gRNA Name: lep gRNA1
Synonyms: trop lep T12
Genomic Target: lep

PAM Sequence: AGG
Sequence: 5' ATGCCAAGGAAGAGCTATAAAGG 3'



Genomic Target tropicalis laevis.S
Identities 23/23 20/23
genome
gRNA
 
5' CCTTTATAGCTCTTCCTTGGCAT 3'
   |||||||||||||||||||||||
3' GGAAATATCGAGAAGGAACCGTA 5'
 
5' CCTTTATAGCTCGCCCTTGGCTT 3'
   ||||||||||||  ||||||| |
3' GGAAATATCGAGAAGGAACCGTA 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr3S:62853534..62853556 20/23 Antisense target lep.S
chr4S:34451192..34451214 20/23 Antisense off-target LOC108714966 [provisional:wwox]
chr8L:23058871..23058893 19/23 Sense off-target atrx.L
chr6L:90551698..90551720 19/23 Antisense off-target XB5863395.L [provisional]
chr6S:13084819..13084841 19/23 Antisense unknown
chr6S:42671366..42671388 19/23 Antisense unknown
chr7S:30565814..30565836 19/23 Sense unknown
chr7S:40971195..40971217 19/23 Antisense unknown
tropicalis 10.0 Chr3:87925111..87925133 23/23 Sense target lep
Chr1:27865046..27865068 19/23 Sense off-target cc2d2a
Chr6:52492812..52492834 19/23 Antisense off-target dnajc13
Chr2:142846009..142846031 20/23 Antisense unknown
Chr1:27280793..27280815 19/23 Sense unknown
Chr7:43002551..43002573 19/23 Sense unknown
Chr9:23119208..23119230 19/23 Antisense unknown
Publications
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