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XB-gRNA-23659399
gRNA Name: lmna gRNA1
Synonyms: trop lmna T2
Genomic Target: lmna

PAM Sequence: AGG
Sequence: 5' CGTGGAAGAGGTGGATCCGGAGG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 23/23 21/23
genomic

gRNA		 5' CCTCCGGATCCACCTCTTCCACG 3' ||||||||||||||||||||||| 3' GGAGGCCTAGGTGGAGAAGGTGC 5' 5' CCTCGGGATCCACCTCTTCCACT 3' |||| ||||||||||||||||| 3' GGAGGCCTAGGTGGAGAAGGTGC 5'
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr8L:117263033..117263055 21/23 Sense target lmna.L
chr3S:7505522..7505544 19/23 Antisense off-target fat2.S
chr2S:67717133..67717155 19/23 Sense off-target l3mbtl3.S
chr8S:96977122..96977144 21/23 Sense off-target LOC108700617
Scaffold20:3908621..3908643 19/23 Sense off-target LOC108703518 [provisional:gpc6]
chr5S:37496761..37496783 19/23 Sense off-target LOC108717827 [provisional:atp5if1]
chr6S:64589959..64589981 20/23 unknown
chr8S:40499942..40499964 20/23 unknown
tropicalis 10.0 Chr8:142920191..142920213 23/23 1 Sense target lmna
Chr2:83385790..83385812 19/23 2 Sense off-target l3mbtl3
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