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XB-gRNA-23659412
gRNA Name:
naa20 gRNA1
Synonyms: trop naa20 T1
Genomic Target: naa20
PAM Sequence: TGG
Sequence: 5' TAACCCATGAGTTCTCCACCTGG 3'
Synonyms: trop naa20 T1
Genomic Target: naa20
PAM Sequence: TGG
Sequence: 5' TAACCCATGAGTTCTCCACCTGG 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 23/23 | 22/23 | |||||
| genomic gRNA |
5' CCAGGTGGAGAACTCATGGGTTA 3' ||||||||||||||||||||||| 3' GGTCCACCTCTTGAGTACCCAAT 5' | 5' CCAGGTGGAGAACTCATGGGCTA 3' |||||||||||||||||||| || 3' GGTCCACCTCTTGAGTACCCAAT 5' | |||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | ||
| laevis 9.2 | chr5S:29279211..29279233 | 22/23 | Antisense | target | naa20.S | ||
| chr7L:84396718..84396740 | 20/23 | Sense | off-target | morn1.L | |||
| chr9_10S:23874419..23874441 | 19/23 | Sense | off-target | usp36.S | |||
| chr3S:69538648..69538670 | 19/23 | Antisense | off-target | sema3e.S | |||
| chr9_10S:23874419..23874441 | 19/23 | Sense | off-target | Xelaev18046477m | |||
| chr9_10S:7188025..7188047 | 19/23 | Antisense | off-target | LOC108702575 [provisional:jph2] | tropicalis 10.0 | Chr5:36942235..36942257 | 23/23 | 1 Antisense | target | naa20 |
| Chr10:33199359..33199381 | 19/23 | 2 Sense | off-target | kif19 | |||
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