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XB-gRNA-23659491
gRNA Name:
zeb2 gRNA1
Synonyms: trop zeb2 T9
Genomic Target: zeb2
PAM Sequence: CGG
Sequence: 5' GTACTTGCAGCGGACTGATACGG 3'
Synonyms: trop zeb2 T9
Genomic Target: zeb2
PAM Sequence: CGG
Sequence: 5' GTACTTGCAGCGGACTGATACGG 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 23/23 | 21/23 | 22/23 | ||||
| genomic gRNA |
5' CCGTATCAGTCCGCTGCAAGTAC 3' ||||||||||||||||||||||| 3' GGCATAGTCAGGCGACGTTCATG 5' | 5' CTGTATCACTCCGCTGCAAGTAC 3' | |||||| |||||||||||||| 3' GGCATAGTCAGGCGACGTTCATG 5' | 5' CCGTATCACTCCGCTGCAAGTAC 3' |||||||| |||||||||||||| 3' GGCATAGTCAGGCGACGTTCATG 5' | ||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | ||
| laevis 9.2 | chr9_10S:51237152..51237174 | 22/23 | Sense | target | zeb2.S | ||
| chr9_10L:55959584..55959606 | 21/23 | Sense | target | zeb2.L | |||
| chr1L:173632273..173632295 | 19/23 | Sense | off-target | LOC108717343 [provisional:tmed7-ticam2] | tropicalis 10.0 | Chr9:78623460..78623482 | 23/23 | 1 Sense | target | zeb2 |
| Chr3:31593576..31593598 | 19/23 | 2 Antisense | off-target | ankhd1 | |||
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