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XB-gRNA-23659702
gRNA Name:
ets1 gRNA1
Synonyms: est1-T1
Genomic Target: ets1
PAM Sequence: GGG
Sequence: 5' GGAGCAGCATTATGTGGCCT 3'
Synonyms: est1-T1
Genomic Target: ets1
PAM Sequence: GGG
Sequence: 5' GGAGCAGCATTATGTGGCCT 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 20/20 | 19/20 | |||||
| genomic gRNA |
5' AGGCCACATAATGCTGCTCC 3' |||||||||||||||||||| 3' TCCGGTGTATTACGACGAGG 5' | 5' AGGGCACATAATGCTGCTCC 3' ||| |||||||||||||||| 3' TCCGGTGTATTACGACGAGG 5' | |||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | ||
| laevis 10.1 | Chr7S:60334165..60334184 | 19/20 | Sense | target | ets1.S | ||
| Chr3S:130097612..130097631 | 16/20 | unknown | tropicalis 10.0 | Chr7:72044997..72045016 | 20/20 | Sense | target | ets1 |
| Chr3:86530797..86530816 | 17/20 | Antisense | off-target | LOC101732997 | |||
| Chr5:70507328..70507347 | 17/20 | unknown | |||||
| Publications | |||||||
| First: |
Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis., Development 2014  |
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| Most recent: | |||||||
| View All Papers | |||||||
