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XB-gRNA-23659703
gRNA Name:
ets1 gRNA2
Synonyms: ets1-T2
Genomic Target: ets1
PAM Sequence: cgg
Sequence: 5' GGTTCAGAGAATTCAGAGGG 3'
Synonyms: ets1-T2
Genomic Target: ets1
PAM Sequence: cgg
Sequence: 5' GGTTCAGAGAATTCAGAGGG 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 20/20 | 20/20 | 20/20 | ||||
| genomic gRNA |
5' CCCTCTGAATTCTCTGAACC 3' |||||||||||||||||||| 3' GGGAGACTTAAGAGACTTGG 5' | 5' CCCTCTGAATTCTCTGAACC 3' |||||||||||||||||||| 3' GGGAGACTTAAGAGACTTGG 5' | 5' CCCTCTGAATTCTCTGAACC 3' |||||||||||||||||||| 3' GGGAGACTTAAGAGACTTGG 5' | ||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | ||
| laevis 10.1 | Chr7L:74683749..74683768 | 20/20 | Antisense | target | ets1.L | ||
| Chr7S:60327278..60327297 | 20/20 | Antisense | target | ets1.S | |||
| Chr6S:102763642..102763661 | 16/20 | Antisense | off-target | chd7.S | |||
| Chr2L:122359377..122359396 | 17/20 | Antisense | off-target | myo16.L | |||
| Chr2L:78866129..78866148 | 17/20 | Sense | off-target | kcnd3.L | |||
| Chr1S:96642018..96642037 | 16/20 | Antisense | off-target | ssbp4.S | |||
| Chr1S:92666589..92666608 | 16/20 | Antisense | off-target | acsbg2.S | |||
| Chr2S:133419240..133419259 | 16/20 | Sense | off-target | marchf9.S | |||
| Chr1S:34853305..34853324 | 17/20 | Sense | off-target | LOC108706622 | |||
| Chr9_10S:44220405..44220424 | 17/20 | Sense | off-target | LOC121399046 | |||
| Chr4L:151170425..151170444 | 16/20 | Antisense | off-target | LOC108704021 | |||
| Chr5S:239436..239455 | 16/20 | Sense | off-target | LOC121394147 | |||
| Chr1L:200582940..200582959 | 18/20 | unknown | |||||
| Chr7S:9819100..9819119 | 17/20 | unknown | |||||
| Chr5L:59159479..59159498 | 17/20 | unknown | |||||
| Chr5L:70641063..70641082 | 17/20 | unknown | |||||
| Chr5S:50923765..50923784 | 17/20 | unknown | |||||
| Chr6L:98443380..98443399 | 16/20 | unknown | |||||
| Chr7S:97947956..97947975 | 16/20 | unknown | |||||
| Chr8L:36416545..36416564 | 16/20 | unknown | |||||
| Chr9_10L:104237565..104237584 | 16/20 | unknown | |||||
| Chr9_10L:69590174..69590193 | 16/20 | unknown | |||||
| Chr4L:111512209..111512228 | 16/20 | unknown | |||||
| Chr5S:135947729..135947748 | 16/20 | unknown | |||||
| Chr6L:19478972..19478991 | 16/20 | unknown | tropicalis 10.0 | Chr7:72040712..72040731 | 20/20 | 1 Antisense | target | ets1 |
| Chr5:30287611..30287630 | 19/20 | 2 Sense | off-target | aplf | |||
| Chr1:131251030..131251049 | 16/20 | 3 Sense | off-target | rorb | |||
| Chr5:79881608..79881627 | 16/20 | 4 Antisense | off-target | prep | |||
| Chr5:49827397..49827416 | 18/20 | 5 | unknown | ||||
| Chr6:20630465..20630484 | 17/20 | 6 | unknown | ||||
| Publications | |||||||
| First: |
Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis., Development 2014  |
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| Most recent: | |||||||
| View All Papers | |||||||
