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Summary Attributions Wiki
XB-GRNA-25874561
gRNA Name: six1 gRNA3
Synonyms: six1 T2
Genomic Target: six1

Sequence: 5' GGGGCAGTGGGCAAGTACA 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 19/19 19/19 18/19
genome
gRNA
 
5' TGTACTTGCCCACTGCCCC 3'
   |||||||||||||||||||
3' ACATGAACGGGTGACGGGG 5'
 
5' TGTACTTGCCCACTGCCCC 3'
   |||||||||||||||||||
3' ACATGAACGGGTGACGGGG 5'
 
5' TGTACTTGCCCACCGCCCC 3'
   ||||||||||||| |||||
3' ACATGAACGGGTGACGGGG 5'
    
Genomic Alignments Position Identity Strand Genomic Target
laevis 9.2 chr8L:79054397..79054415 19/19 Sense target six1.L
chr8S:7826490..7826508 18/19 Sense target six1.S
chr8L:91882773..91882791 18/19 Sense off-target npas3.L
Scaffold31:2223340..2223358 17/19 Antisense off-target parp12.L
Scaffold31:2224156..2224174 17/19 Antisense off-target parp12.L
chr4L:126030256..126030274 17/19 Sense off-target LOC108714640 [provisional:cand1]
chr8L:119190195..119190213 17/19 Sense off-target LOC108699478 [provisional:trim46]
Scaffold31:2223340..2223358 17/19 Antisense off-target LOC108704344 [provisional:zc3hav1l]
chr6S:40596793..40596811 16/19 Antisense off-target LOC108719962 [provisional:rbms3]
chr6S:35467743..35467761 16/19 Antisense off-target LOC108719943 [provisional:ankrd28]
chr4S:10054235..10054253 16/19 unknown
tropicalis 10.0 Chr8:102416079..102416097 19/19 Sense six1
Chr5:19213518..19213536 19/19 Sense six2
Chr4:131814771..131814789 17/19 Sense cand2
Chr2:180668381..180668399 16/19 Antisense ilk
Chr2:180668350..180668368 16/19 Antisense ilk
Chr2:180668412..180668430 16/19 Antisense ilk
Chr2:180668443..180668461 16/19 Antisense ilk
Chr2:180668474..180668492 16/19 Antisense ilk
Chr2:180668505..180668523 16/19 Antisense ilk
Chr2:180668536..180668554 16/19 Antisense ilk
Chr2:180668567..180668585 16/19 Antisense ilk
Chr2:180668660..180668678 16/19 Antisense ilk
Chr2:180668752..180668770 16/19 Antisense ilk
Chr2:180668843..180668861 16/19 Antisense ilk
Chr2:180668934..180668952 16/19 Antisense ilk
Chr4:125821923..125821941 16/19 Sense usp4
Chr3:127316585..127316603 16/19 Antisense nkx2-6
Chr8:59492708..59492726 16/19 Antisense kiaa1210
Chr7:29137338..29137356 18/19
Chr2:156371694..156371712 16/19
Chr5:107641203..107641221 16/19
Publications
???morpholino.displaySummary.papersFirst??? Generation of a new six1-null line in Xenopus tropicalis for study of development and congenital disease., Genesis 2021          
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