Fig. 4. Whole-mount in situ hybridization of XGAP-43 during Xenopus laevis embryogenesis. Numbers in the left column represent the approximate stage of embryos in each row. Embryos in columns (A), (B), (D) and (E) were incubated with a full-length antisense XGAP-43 probe and those in columns (C) and (F) with full-length sense XGAP-43. Lateral views of XGAP-43 expression are shown in columns (A) and (D-F), and dorsal views in column (B). XGAP-43 transcripts were first observed around stage 22 where they appeared as two parallel stripes on either side of the midline, and also in bilateral regions of the prospective forebrain/midbrain border. In later stages, XGAP-43 expression was observed in a complex spatiotemporal pattern throughout the nervous system. E: magnification of CNS structures expressing XGAP-43 in a stage 34 embryo: 1 = brainstem; 2 = hindbrain; 3- cerebellum; 4 = midbrain; 5 = pineal gland; 6 = forebrain (olfactory region); 7 = diencephalon; 8 = optic vesicle; 9 = trigeminal ganglia; 10 = otic capsule. Structures are based on previous descriptions of Xenopus neuroanatomy [16,17,25]. Probe preparation and whole-mount in situ hybridization were performed as described [26].
Image published in: Shain DH et al. (1995)
Copyright © 1995. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| gap43.L | b-50, gap43-a, gap43-b, neuromodulin, pp46 | X. laevis | Throughout NF stage 22 | olfactory placode neural tube posterior neural tube |
| gap43.L | b-50, gap43-a, gap43-b, neuromodulin, pp46 | X. laevis | Throughout NF stage 26 | central nervous system brain spinal cord olfactory placode diencephalon trigeminal placode |
| gap43.L | b-50, gap43-a, gap43-b, neuromodulin, pp46 | X. laevis | Throughout NF stage 29 and 30 | central nervous system brain spinal cord diencephalon trigeminal nerve |
| gap43.L | b-50, gap43-a, gap43-b, neuromodulin, pp46 | X. laevis | Throughout NF stage 33 and 34 | pineal gland central nervous system brain spinal cord trigeminal nerve hindbrain cerebellum midbrain eye diencephalon olfactory bulb |
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