Fig. 7. xMIDs function in the developing brain and other epithelial organogeneses. (A-J) Transverse sections through the brain (A,B), optic vesicle (C,D), cement gland (E,F), pronephros (G,H) and foregut (I,J) of Xenopus embryos injected with control-Mo (A,C,E,G,I) or xMID-Mo (B,D,F,H,J), and stained with phalloidin (green), anti-laminin antibody (magenta), or anti-Flag antibody (gray). (A,B) Insets show stage 40 embryos stained for the pan-neural marker Xen1. Lateral views: anterior to the left, dorsal to the top. Arrowheads indicate head defects in the xMID morphant. (B,D) Arrowheads indicate non-continuous and attenuated laminin staining in the basal lamina. (E,F) Brackets indicate the lengths of Flag-positive cells. (G) Asterisks indicate tubular structures of the pronephros. (I,J) Asterisks indicate luminal structures of the foregut. Arrowheads indicate the lack of apical actin assembly in the xMID morphant cells. (K,L) Transverse sections through the brain of control-Mo-injected (K) and xMID-Mo-injected (L) embryos, stained with anti-active caspase 3 (left) and anti-Flag (right) antibodies. Dashed line delimits the ventricle.
Image published in: Suzuki M et al. (2010)
Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| lamb2.L | lams | X. laevis | Throughout NF stage 26 | head brain foregut pronephric kidney cement gland primordium optic vesicle gut epithelium epithelium |
| en1.L | en-1, en1-a, en1-b, eng1, engrailed 1, engrailed-1, xen1 | X. laevis | Throughout NF stage 40 | brain forebrain midbrain hindbrain spinal cord neural tube anterior neural tube neuroectoderm eye retina |
| casp3.2.S | Apopain, CASP-3, caspase 3, caspase-3, xCPP32 | X. laevis | Throughout NF stage 25 | brain epithelium |
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