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Fig. 2. Whole-mount in situ hybridization of selected array clones. (A) Xenopus embryos at early gastrula (st. 10), tailbud (st. 30) stage and dissected whole gut (st. 43) were hybridized with antisense probe for Fz7/clone 10B11. Arrowheads indicate expression in the endoderm; the dotted line depicts the dorsal blastopore lip. Staining in endodermal derivatives, such as the hepatic and pancreatic regions, is indicated by a bracket in the middle panel. (B) Embryos at early gastrula (st. 10), tadpole (st. 35) stage and dissected whole gut (st. 43) were hybridized with HDGF/clone 7C5. Arrowheads point to expression in the endoderm; and the bracket in the middle panel indicates endodermal derivatives, such as the hepatic and pancreatic regions. (C) Embryos at gastrula (st. 10.5), tadpole (st. 37) stage and dissected whole gut (st. 42) were hybridized with clone 7G9 [EST similar to hypothetical protein (BG410109)] indicating expression in the ectoderm and hepatic rudiment (bracket in the middle panel). (D) Embryos at gastrula (st. 10), tadpole (st. 36) stage and dissected whole gut (st. 42) were hybridized with clone 8C11 [EST similar to hypothetical protein KIAA0592 (BG410148)] showing expression in the dorsal ectoderm, brain, pronephros, liver and duodenum. (E) Embryos at gastrula (st. 10), early tailbud (st. 27) stage and dissected whole gut (st. 42) were hybridized with clone 7B7 [EST similar to hypothetical protein (BC094159)] indicating expression in the ectoderm, brain, branchial arches and pancreas. (F-J) In situ hybridizations on serial sections of gastrula and neurula stage embryos using Xenopus TGIF2 (xTGIF2) and Gata5 probes show expression of both genes in a subset of endodermal cells. xTGIF2 is also strongly expressed in the ectoderm and mesoderm. (F,F′) In situ hybridization on section of stage 10 embryo using antisense probe for TGIF2/clone 8B1 (* indicates the dorsal side). Red dashed box outlines the TGIF2-positive endodermal region, which is magnified in F′. Arrowheads point to endodermal cells. (G) Gata5 in situ hybridization on section of stage 10 embryo. Arrowheads point to endodermal cells. (H,H′) In situ hybridization on section of stage 10.5 embryo shows enriched staining in dorsal endodermal cells (* indicates the dorsal side). Red dashed box outlines the TGIF2-positive endodermal region, which is magnified in H'. Arrowheads point to endodermal cells. (I,I′) In situ hybridization on neurula stage whole (I) and sectioned (I′) embryos shows TGIF2 staining in anterior endodermal cells (red dashed box in I′). (J) Gata5 in situ hybridization on section of stage 17 embryo. (K) Dissected whole gut (st. 43) stained with TGIF2 probe shows expression in the pancreatic rudiment. a, anterior; d, duodenum; lv, liver; pa, pancreas; p, posterior.

Image published in: Spagnoli FM and Brivanlou AH (2008)

Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneSynonymsSpeciesStage(s)Tissue
fzd7Fz-7, Xfz7, frizzled-7, frizzled7, frizzled 7, frz7, frz-7, fz7X. tropicalisThroughout NF stage 10dorsal
vegetal
endoderm
ectoderm
fzd7.LFz-7, Xfz7, frizzled-7, frizzled7, frizzled 7, frz7, frz-7, fz7X. laevisThroughout NF stage 29 and 30endoderm
pancreas
liver primordium
pronephric duct
pronephric kidney
cephalic nerve
trigeminal nerve
facial nerve
eye
tail bud
head region
thyroid primordium
alimentary system
foregut
fzd7.LFz-7, Xfz7, frizzled-7, frizzled7, frizzled 7, frz7, frz-7, fz7X. laevisThroughout NF stage 43foregut
alimentary system
liver and biliary system
pancreas
hdgf.LX. laevisThroughout NF stage 10endoderm
ectoderm
mesoderm
blastocoel roof
blastocoel floor
endomesoderm
hdgf.LX. laevisThroughout NF stage 35 and 36brain
eye
otic vesicle
somite
hypaxial muscle
foregut
lateral plate mesoderm
pancreas
branchial arch
alimentary system
posterior neural tube
pharyngeal arch
liver and biliary system
anterior dorsal lateral plate region
hdgf.LX. laevisThroughout NF stage 43pancreas
duodenum
alimentary system
c19orf25.LX. laevisThroughout NF stage 10.5ectoderm
marginal zone
mesoderm
c19orf25.LX. laevisThroughout NF stage 37 and 38endoderm
brain
pharyngeal arch
otic vesicle
liver primordium
foregut
hindgut
head
c19orf25.LX. laevisThroughout NF stage 42liver
alimentary system
washc2a.Lfam21aX. laevisThroughout NF stage 10dorsal
ectoderm
washc2a.Lfam21aX. laevisThroughout NF stage 35 and 36endoderm
brain
eye
pharyngeal arch
foregut
pronephric kidney
lateral plate mesoderm
washc2a.Lfam21aX. laevisThroughout NF stage 42liver
duodenum
alimentary system
foregut
rcc2.LTD-60, telophase disc 60 kDa proteinX. laevisThroughout NF stage 10dorsal
ectoderm
rcc2.LTD-60, telophase disc 60 kDa proteinX. laevisThroughout NF stage 27cranial neural crest
mandibular crest
hyoid crest
anterior branchial crest
posterior branchial crest
tail bud
anterior neural tube
brain
hindbrain
otic vesicle
pronephric mesenchyme
foregut
endoderm
rcc2.LTD-60, telophase disc 60 kDa proteinX. laevisThroughout NF stage 42pancreas
dorsal pancreatic bud
ventral pancreatic bud
foregut
tgif2.Lxtgif2, TGFb-induced2, TGFb induced2X. laevisThroughout NF stage 10blastocoel roof
endoderm
mesoderm
endomesoderm
tgif2.Lxtgif2, TGFb-induced2, TGFb induced2X. laevisThroughout NF stage 17neural plate
ventral
mesoderm
anterior
endodermal cell
neuroectoderm
neuroectoderm outer layer
tgif2.Lxtgif2, TGFb-induced2, TGFb induced2X. laevisThroughout NF stage 43endoderm
pancreas
duodenum
alimentary system
first pancreatic bud
second pancreatic bud
foregut
gata5.SxGATA-5, xGATA5, xGATA-5a, xGATA5a, xGATA-5b, xGATA5b, GATA-5, gata-4a, gata5-b, gata5-aX. laevisSometime during NF stage 10 to NF stage 10.5endodermal cell
endomesoderm
gata5.SxGATA-5, xGATA5, xGATA-5a, xGATA5a, xGATA-5b, xGATA5b, GATA-5, gata-4a, gata5-b, gata5-aX. laevisThroughout NF stage 17anterior
ventral
mesoderm
endodermal cell

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