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Fig. 5. Comparison of the localization of XMEF2C, Xscleraxis, betaig-h3, tenascin C and tenomodulin mRNA by whole-mount in situ hybridization. (A) Betaig-h3 mRNA begins to be expressed in connective tissue associated with hypaxial muscle at stage 41. (B) XMEF2C, Xscleraxis, betaig-h3, tenascin C and tenomodulin mRNA are largely colocalized at stage 45 in head region (ventral view, top) intersomitic space as indicated by staining between the convex myotome (dorsal view, middle). Connective tissue associated with hypaxial muscles expresses XMEF2C, Xscleraxis, betaig-h3 and tenascin C, but we cannot detect tenomodulin mRNA (lateral view, bottom). (C) Col1a2, col5a1 and col12a1 mRNA is also expressed in intersomitic space at stage 45 (D) Comparison of MEF2C and scleraxis mRNA accumulation 3 days after feeding. MEF2C mRNA is always expressed at this stage while scleraxis mRNA appears stronger. Arrows indicate connective tissue associated with hypaxial muscles expression. Numbers indicate developmental stages.

Image published in: della Gaspera B et al. (2009)

Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V.

GeneSynonymsSpeciesStage(s)Tissue
mef2c.LXMEF2CX. laevisThroughout NF stage 45head region
muscle
tendon
myotome
somite
hypaxial muscle
connective tissue
scx.Lscleraxis, xscleraxisX. laevisThroughout NF stage 45muscle
head region
tendon
somite
hypaxial muscle
connective tissue
tnc.LLOC108700035, tenascin, tenascin C, tenascin-CX. laevisThroughout NF stage 45hypaxial muscle
connective tissue
tendon
tnmd.SX. laevisThroughout NF stage 45tendon
somite
connective tissue
tgfbi.Lbetaig-h3, bigh3, cdb1, cdg2, cdgg1, csd, csd1, csd2, csd3, ebmd, keratoepithelin, lcd1, LOC108712407, xtgfbiX. laevisSometime during NF stage 41 to NF stage 42tendon
somite
hypaxial muscle
connective tissue
col1a2.LMGC52958, procollagen, type I, alpha 2X. laevisThroughout NF stage 45tendon
somite
connective tissue
col5a1.LX. laevisThroughout NF stage 45muscle
tendon
somite
connective tissue
col12a1.LX. laevisThroughout NF stage 45muscle
tendon
somite
hypaxial muscle
connective tissue

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