Fig. 1 F G

Angerilli A et al. (2023)

Xla Wt + kmt5c MO + kmt5b MO (H1/H2 double KD)

NF28 (immunohistochemistry)

Anatomical Phenotype
abnormal multiciliated cell abnormally decreased number of cilium in the ciliated cell

Expression Phenotype
decreased amount tuba4b.L expression in multiciliated epidermal cell

Fig. S4: A r2c2; Dr2 c2

Angerilli A et al. (2023)

Xla Wt + kmt5c MO + kmt5b MO (H1/H2 double KD)

NF28 (in situ hybridization)

Anatomical Phenotype
abnormal embryo morphology abnormal multiciliated cell morphology abnormally decreased number of cilium in the ciliated cell

Expression Phenotype
decreased amount tuba4b.L expression in multiciliated epidermal cell

Fig. 5 A: r2c2

Angerilli A et al. (2023)

Xtr Wt + kmt5b MO (suv4-20h1 NF4)

NF35/36 (in situ hybridization)

Anatomical Phenotype
abnormal embryo morphology abnormal multiciliated cell morphology abnormally decreased number of cilium in the ciliated cell

Expression Phenotype
decreased amount tuba4b expression in multiciliated epidermal cell

Fig. 5. A r4c2, B

Angerilli A et al. (2023)

Xtr Wt + kmt5b MO + kmt5c MO (H1/H2KD)

NF35/36 (in situ hybridization)

Anatomical Phenotype
abnormal embryo morphology abnormal main body axis morphology abnormal multiciliated cell morphology abnormally decreased number of cilium in the ciliated cell

Expression Phenotype
absent tuba4b expression in multiciliated epidermal cell

Fig. S5 r_2,c_2

Sempou E et al. (2022)

Xtr Wt + kcnh6 CRISPR (400pg)

NF17 (immunohistochemistry) tuba4b

Expression Phenotype
decreased amount tuba4b expression in gastrocoel roof plate

Fig. 6 D

Bharathan NK and Dickinson AJG (2019)

Xla Wt + dsp MO

NF19-20 (immunohistochemistry)

Anatomical Phenotype
abnormal epidermis morphology abnormally localised microtubule

Expression Phenotype
mislocalised tuba4b.L expression in epidermis

skin disease

Fig. S 2 A, B

Sempou E et al. (2018)

Xtr Wt + fgfr4 CRISPR

NF17 (immunohistochemistry)

Anatomical Phenotype
abnormal gastrocoel roof plate morphology abnormal left-right organizer morphology abnormally decreased number of cilium in the left-right organizer

Expression Phenotype
decreased amount tuba4b expression in left-right organizer

Fig. 4. H. J.

Zhou F et al. (2015)

Xla Wt + gmnc MO

NF29/30-29 and 30 (immunohistochemistry)

Anatomical Phenotype
abnormally decreased number of ciliated cell

Expression Phenotype
decreased amount tuba4b.L expression in cilium

Fig. S4. B. C

Zhou F et al. (2015)

Xla Wt + Hsa.gmnc

NF29/30-29 and 30 (immunohistochemistry)

Expression Phenotype
increased amount tuba4b.L expression in multiciliated cell

fig.4.a, b

Haremaki T et al. (2015)

Xla Wt + htt MO

NF29/30 (immunohistochemistry)

Expression Phenotype
decreased amount tuba4b.L expression in multiciliated cell