Fig.3.D,E

Ossipova O et al. (2020)

Xla Wt + pnhd + animal cap explant

NF10.5-11 (RT-PCR)

Expression Phenotype
increased amount wnt8a.L expression in the animal cap increased amount tbxt.S expression in the animal cap increased amount vegt.L expression in the animal cap

Fig.4.D

Ossipova O et al. (2020)

Xla Wt + pnhd-FLAG + animal cap explant

NF10.5-11 (RT-PCR)

Expression Phenotype
increased amount wnt8a.L expression in the animal cap increased amount cdx4.L expression in the animal cap increased amount hoxd1.L expression in the animal cap increased amount tbxt.S expression in the animal cap increased amount fgf8.L expression in animal cap

Fig.4.B,C

Ossipova O et al. (2020)

Xla Wt + pnhd-FLAG + animal cap explant

NF11 (RNA-Seq)

Expression Phenotype

increased amount wnt8a.L expression in the animal cap
increased amount cdx1.L expression in the animal cap
increased amount cdx4.L expression in the animal cap
increased amount esr-5.L expression in the animal cap
increased amount flrt3.L expression in the animal cap
increased amount hoxd1.L expression in the animal cap
increased amount msgn1.L expression in the animal cap
increased amount myf5.L expression in the animal cap
increased amount tbxt.S expression in the animal cap
increased amount vegt.L expression in the animal cap
decreased amount ccne2.S expression in the animal cap
decreased amount foxi4.2.L expression in the animal cap
decreased amount nedd9.L expression in the animal cap
decreased amount nedd9.S expression in the animal cap
decreased amount xepsin.L expression in the animal cap
increased amount fgf8.L expression in animal cap

Fig.3.S1.B

Mukherjee S et al. (2020)

Xtr Wt + ctnnb1 MO

NF10.5 (in situ hybridization)

Expression Phenotype

increased amount wnt8a expression in the animal cap
increased amount wnt8a expression in blastocoel floor
increased amount wnt8a expression in ectoderm
increased amount wnt8a expression in endoderm
increased amount wnt8a expression in ventral marginal zone
absent fst expression in dorsal involuting marginal zone
absent fst expression in involuted dorsal mesoderm
decreased amount admp expression in axial mesoderm
decreased amount admp expression in dorsal endomesoderm
decreased amount admp expression in endoderm
increased amount ventx3.1 expression in animal cap
increased amount ventx3.1 expression in blastocoel floor
increased amount ventx3.1 expression in endoderm

Fig.1.S1.D

Mukherjee S et al. (2020)

Xtr Wt + sox17a MO + sox17b.1/b.2 MO

NF10.5 (in situ hybridization)

Expression Phenotype

increased amount wnt8a expression in the animal cap
increased amount wnt8a expression in blastocoel floor
increased amount wnt8a expression in ectoderm
increased amount wnt8a expression in ventral marginal zone
decreased amount admp expression in dorsal involuting marginal zone
decreased amount dhh expression in blastopore lip
decreased amount gata4 expression in endoderm
decreased amount otx2 expression in dorsal endomesoderm
decreased amount otx2 expression in dorsal involuting marginal zone
increased amount nodal1 expression in endoderm
increased amount nodal1 expression in marginal zone
increased amount nodal1 expression in ventral marginal zone

Fig.4.D

Ossipova O et al. (2020)

Xla Wt + pnhd + animal cap explant

NF12 (RT-PCR)

Expression Phenotype
increased amount wnt8a.L expression in the animal cap increased amount cdx4.L expression in the animal cap increased amount hoxd1.S expression in the animal cap increased amount tbxt.S expression in the animal cap increased amount fgf8.L expression in animal cap

Fig.S4.C

Ossipova O et al. (2020)

Xla Wt + pnhd MO

NF11-12 (in situ hybridization)

Expression Phenotype
decreased amount wnt8a.L expression in marginal zone

Fig.S4.B

Ossipova O et al. (2020)

Xla Wt + pnhd MO

NF11-12 (in situ hybridization)

Expression Phenotype
decreased amount wnt8a.L expression in marginal zone

Fig.S4.F

Ossipova O et al. (2020)

Xla Wt + pnhd MO + marginal zone explant

NF10.5 (RT-PCR)

Expression Phenotype
decreased amount wnt8a.L expression in marginal zone decreased amount hoxd1.S expression in marginal zone decreased amount msgn1.L expression in marginal zone decreased amount tbxt.S expression in marginal zone

Fig.S4.D

Ossipova O et al. (2020)

Xla Wt + pnhd-FLAG

NF11-12 (in situ hybridization)

Expression Phenotype
increased amount wnt8a.L expression in marginal zone