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XB-PHENO-23096

Xla Wt + Mmu.lef1-Mmu.ctnnb1-GR + fzd7 MO + DEX, Zhang Z et al. (2013)

Experiment + Assay: Xla Wt + Mmu.lef1-Mmu.ctnnb1-GR + fzd7 MO + DEX + NF20 (in situ hybridization)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection fzd7 MO3 fzd7.L
fzd7.S
 NF stage 6 (32-cell) dorsal animal hemisphere blastomere
mRNA injection Tg(Mmu.lef1-Mmu.ctnnb1-GR) ctnnb1.S
ctnnb1.L
 NF stage 6 (32-cell) dorsal animal hemisphere blastomere
chemical dexamethasone
NF stage 11 whole organism
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 20  
Expression Phenotypes (1):
decreased amount hhex.L expression in foregut
Source: fig.6.k
Article: XB-ART-46881, PubMed