XB-PHENO-23391
Xla Wt + nog + sipa1l3 MO + animal cap explant, Rothe M et al. (2017)
| Experiment + Assay: |
Xla Wt + nog + sipa1l3 MO + animal cap explant +
NF13 (real time PCR)
|
| Manipulations: |
| Manipulation Type |
Reagent |
Target Gene |
Stage |
Treatment Area |
|
morpholino injection
|
sipa1l3 MO1
|
sipa1l3.L
sipa1l3.S
|
NF stage 2 (2-cell)
|
blastomere
|
|
mRNA injection
|
nog.L
|
nog.S
nog.L
|
NF stage 2 (2-cell)
|
blastomere
|
|
explant
|
|
|
NF stage 8
|
animal cap
|
|
| Conditions: |
| Assay Type |
Assay Stages |
Antibody |
|
real time PCR
|
NF stage 13
|
|
|
|
|
Expression Phenotypes (1):
|
|
increased amount
axin2.L
expression in animal cap
|
|
