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XB-PHENO-25627

Xla Wt + pnhd + animal cap explant, Ossipova O et al. (2020)

Experiment + Assay: Xla Wt + pnhd + animal cap explant + NF10.5-11 (RT-PCR)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection pnhd.L pnhd.L
 NF stage 2 (2-cell) blastomere
explant  
NF stage 10 animal cap
Conditions:
Assay Type Assay Stages Antibody
RT-PCR NF stage 10.5 to NF stage 11  
Expression Phenotypes (3):
increased amount tbxt.S expression in the animal cap
increased amount vegt.L expression in the animal cap
increased amount wnt8a.L expression in the animal cap
Source: Fig.3.D,E
Article: XB-ART-57304, PubMed