XB-PHENO-25628
Xla Wt + Xla.pnhd-FLAG + animal cap explant, Ossipova O et al. (2020)
| Experiment + Assay: |
Xla Wt + Xla.pnhd-FLAG + animal cap explant +
NF10.5-11 (RT-PCR)
|
| Manipulations: |
| Manipulation Type |
Reagent |
Target Gene |
Stage |
Treatment Area |
|
mRNA injection
|
Tg(Xla.pnhd.L-FLAG)
|
pnhd.L
|
NF stage 2 (2-cell)
|
blastomere
|
|
explant
|
|
|
NF stage 9
|
animal cap
|
|
| Conditions: |
| Assay Type |
Assay Stages |
Antibody |
|
RT-PCR
|
NF stage 10.5
to NF stage 11
|
|
|
|
|
Expression Phenotypes (1):
|
|
increased amount
tbxt.S
expression in the animal cap
|
|
