XB-PHENO-25707
Xtr Wt + kcnh6 CRISPR (400pg), Sempou E et al. (2022)
| Experiment + Assay: |
Xtr Wt + kcnh6 CRISPR (400pg) +
NF10 (in situ hybridization)
|
| Manipulations: |
| Manipulation Type |
Reagent |
Target Gene |
Stage |
Treatment Area |
|
CRISPR
|
kcnh6 gRNA1
|
kcnh6
|
NF stage 1
|
whole organism
|
|
| Conditions: |
| Assay Type |
Assay Stages |
Antibody |
|
in situ hybridization
|
NF stage 10
|
|
|
|
|
Expression Phenotypes (7):
|
|
absent
myod1
expression in dorso-lateral marginal zone
|
|
increased amount
pou5f3.1
expression in embryo
|
|
decreased amount
pou5f3.3
expression in embryo
|
|
decreased amount
sox2
expression in dorsal side
|
|
decreased amount
trim33
expression in animal cap
|
|
decreased amount
trim33
expression in endomesoderm
|
|
increased amount
ventx1.2
expression in embryo
|
|
Source:
Fig. 4a,b, e, f, k, l, m, n
