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Search Transgenic Constructs

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Transgene Name Description
Tg(sox5-GA-FLAG)

This construct contains the coding sequence of sox5 fused to a mutated form of the mouse glucocortic...
Tg(sox5-GA-VP16-FLAG)

This construct contains the coding sequence of sox5 fused to a mutated form of the mouse glucocortic...
Tg(sox5-VP16-FLAG)

This construct contains the coding sequence of sox5 fused to the VP16 transactivation domain and a F...
Tg(sox5-GR-FLAG)

This construct contains the coding sequence of sox5 fused to the mouse glucocorticoid receptor bindi...
Tg(sox5-EnR-FLAG)

This construct contains the coding sequence of sox5 fused to the Drosophila engrailed repressor doma...
Tg(eef1a1:GFP)

elongation factor one (eefa1a) promoter driving GFP
Tg(CAG:KikGR)

CAG promoter driving green-to-red photoconvertible fluorescent protein, Kikume Green-Red (KikGR)
Tg(Dre.noto:GFP)

Zebrafish (Dre) floating head (flh) promoter driving GFP. Note flh renamed 'notochord homebox'. In X...
Tg(gata1:GFP)

GATA-1 promoter and first intron driving GFP
Tg(HA-ER-Ppo.iPpoi)

HA tagged and tamoxifen inducible form of iPpoi a nuclear, intron-encoded homing endonuclease from P...
Tg(Xtr.slc26a4:eGFP)

slc26a4 (aka pendrin) PCR was amplified from X. tropicalis genomic library using one primer correspo...
Tg(nkx2-5:GFP)

Tg(Xtr.dio3:GFP)

1448 bp X. tropicalis dio3 promoter driving GFP . The DNA plasmid construct used corresponds to a c...
Tg(Hsa.foxd4)

Construct containing the coding sequence for the human Foxd4 gene.
Tg(tbx6-VP16)

Construct encoding the Tbx6 protein fused to the VP16 activator domain.
Tg(tbx6-VP16-GR)

Construct encoding a hormone inducible form of Tbx6 protein fused to the VP16 activator domain. Horm...
Tg(tbx6-EnR)

Construct encoding a hormone inducible form of Tbx6 protein fused to the drosophila engrailed repres...
Tg(Dre.lbx1)

This construct contains the Danio rerio lbx1 gene subcloned from I.M.A.G.E. EST (GenBank AL831789).
Tg(dhcr7W149X)

Form of dhcr7 bearing W149X substitution which completely lacks the sterol sensing domain (SSD).
Tg(HA-myod1)

This is an HA tagged form of Xenopus tropicalis myod1.
Tg(HA-myod1)

This is an HA tagged form of Xenopus tropicalis myod1.
Tg(Mmu.kdm5bH499A_del)

Catalytically inactive Mouse KDM5B (aa1-770) cloned using the p-Entry cloning system into a pCS2+ pl...
Tg(h3-3a.LK4M)

Dominant negative mutant of h3-3a.L cloned into pCS2+ plasmid with 6 C-terminal HA-tags. The mutatio...
Tg(myl7:Gal4)

A construct in which the myl7 promoter drives the expression of GAL4. This should produced GAL4 expr...
Tg(UAS:Hsa.adprhl1-Xla.adprhl1)

This construct expresses a fusion of the human and Xenopus adprhl1 genes under the UAS responsive pr...
Tg(six1-VP16)

To generate a chimeric transactivating six1 construct, the Six domain plus the homeodomain (SDHD; a...
Tg(six1-EnR)

To generate a chimeric repressive six1 construct, the Six domain plus the homeodomain (SDHD; amino a...
Tg(six1-GR)

To generate a hormone-inducible Six1expression vector (pCS2+-GR-myc-Six1), the full open-reading fra...
Tg(irx1-VP16)

To prepare the irx1-VP16 construct, the VP16Mix.1 DNAs were used (Lemaire et al., 1998). The Mix.1 ...
Tg(irx1-EnR-hGR)

The inducible irx1 in which the irx1 homeodomain was fused to the Drosophila Engrailed repressor ele...
Tg(dnlifr)

lif receptor mutant (Δlifr) cDNA sequences where the intra-cytoplasmic domain has been deleted were ...
Tg(six1-V17E)

A mutation associated with BOS/BOR was introduced into the Xenopus laevis pCS2-Six1 plasmid (Brugman...
Tg(six1-R110W)

A mutation associated with BOS/BOR was introduced into the Xenopus laevis pCS2-Six1 plasmid (Brugman...
Tg(six1-W122R)

A mutation associated with BOS/BOR was introduced into the Xenopus laevis pCS2-Six1 plasmid (Brugman...
Tg(six1-Y129C)

A mutation associated with BOS/BOR was introduced into the Xenopus laevis pCS2-Six1 plasmid (Brugman...
Tg(dnbmpr1a)

A dominant-negative bmpr1a. Also known as Tg(DN-bmpr1a).
Tg(Xla.cd63)Y237A

an endosome-excluded version of cd63, the full-length X. laevis L-homeolog was isolated and the lyso...
Tg(ets2-FLAG)

A construct encoding ets2 triple tagged with FLAG sequences.
Tg(Xla.pnhd.L-FLAG)

pCS2-Flag-Pnhd plasmid was generated by PCR from the X. laevis DNA clone for pnhd.L (accession numbe...
Tg(Hsa.DLG5Arg249Trp)

We generated in vitro capped mRNA of variant human DLG5 from sequences (BC146794) cloned into the p...
Tg(hhex:rtTA;TRE:dnRab11a-GFP)

Construct expressing a dominant negative Rab11 in the foregut, under the hhex promoter, upon inducti...
Tg(Hsa.DSPdel)

A mutant form of the human Dsp. This construct is missing the C terminal domains of Dsp which impair...
Tg(ptpn11N308D)

missense mutation in PTPN11 ( also called SHP-2) from Nonnan Syndrome patients. Site-directed mutage...
Tg(ptpn11N308D,C459G)

Site-directed mutagenesis of ptpn11 ( also called SHP-2) with substitution at N308D to make double ...
Tg(ptpn11D61G)

mutation found in gene SHP-2/ptpn11 from Noonan Syndrome patients that lead to heart defects.
Tg(ptpn11Q79R)

mutation found in gene SHP-2/ptpn11 from Noonan Syndrome patients that lead to heart defects.
Tg(ptpn11E76A)

Site-directed mutagenesis of ptpn11 ( also called SHP-2) with substitution at E76A. Mutation found i...
Tg(ptpn11C459G)

Site-directed mutagenesis of ptpn11 ( also called SHP-2) with substitution at C459G. Mutation found ...
Tg(foxh1-VP16-GR)

This is a fusion construct consisting of the forkhead DNA-binding domain containing fragment (61-365...
Tg(esr1-GR)

X. laevis ESR1 was sub-cloned into pCS2+ 6myc-GR vector

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