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Search Transgenic Constructs

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Transgene Name Description
Tg(Hsa.NUP188)

human NUP188
Tg(dnHsa.SQSTM1)

A synthetic p62/SQSTM1 peptide mimicking the interaction sequence thus identified, p62 {346-388} (a...
Tg(Hsa.SQSTM1)

A construct encoding the human p62/SQSTM1 protein
Tg(Hsa.krasV12 : GFP)

Human kRasV12 from Addgene cloned into N-GFP/pCS2+
Tg(Hsa.myc:GFP)

Human cMYC-IRES-GFP from Addgene cloned into N-GFP/PCS2+.
Tg(Hsa.rhoaQ63L:GFP)

Constitutively active rhoa in pcDNA3-EGFP vector backone. Addgene 12968
Tg(Mmu.grem1)

Wild type murine grem1.
Tg(dnspry2)

A dominant negative form of sprouty2 with a Y55F substitution.
Mmu.wnt1del284

Mmu.wnt1ins285

contains a 4 bp insertion within the wnt-1 open reading frame, resulting in a frame shift at amino a...
Tg(Hsa.NEK2)

Full length human NEK2 including UTRs (MGC:49922) is in a pCMV.Sport.6 backbone
Tg(dnfzd8)

ECD8 construct in pXT7 vector (Dominguez et al. 1995) was generated by PCR from the Xfz8 plasmid (I...
Tg(dndvl1)

The Xdd1 construct has an internal deletion of the DNA fragment corresponding to amino acids 301–38...
Tg(Hsa.ZNRF3)

Human ZNRF3 gene.
Tg(Hsa.FADD)

Human form of the fadd gene.
Tg(myc-myb)

A myc tagged form of myb. Also known as MT-myb.
Tg(Dre.mpeg1:mCherry)Amaya

A transgenic reporter line showing mCherry expression driven by the zebrafish mpeg1 promoter. This l...
Tg(fermt2Q614A_W615A)-myc

This is a myc tagged mutant form of fermt2 which has substitutions in the F3 subdomain interfering w...
Tg(Hsa.GLI2)

Construct containing sequence for the human GLI2 protein.
Tg(Hsa.GLI2V104M)

Construct bearing a variant of human GLI2 with a missense mutation, V104M, 310G>A .
Tg(Hsa.GLI2D88N)

Construct with missense mutation of human GLI2,D88N, 262G>A .
Tg(Hsa.GLI2N273S)

Construct bearing a variant of human GLI2 with a missense mutation, N273S, 818 A>G .
Tg(Hsa.GLI2K410R)

Construct bearing a polymorphic variant of human GLI2, K410R, 1229 A>G.
Tg(Hsa.GLI2del)

Construct bearing a variant of human GLI2 with a deleted exon5.
Tg(Hsa.GLI2P595A_R597G)

Construct bearing a variant of human GLI2, P595A_R597G.
Tg(Hsa.GLI2A638T)

Construct bearing a variant of human GLI2, A638T.
Tg(Hsa.GLI2A828S)

Construct bearing a variant of human GLI2, A828S.
Tg(Hsa.SHOX-nog)

Construct containing fused type 1 wild type SHOX 5′-UTR to the coding sequence of Xenopus noggin. In...
Tg(Hsa.SHOXtm-nog)

Construct with mutated human SHOX 5'-UTR, with 7 uAUG sites mutated, fused to Xenopus noggin (nog) c...
Tg(Hsa.SHOXdel-nog)

Construct containing fused type 2 wild type SHOX 5′-UTR to the coding sequence of Xenopus noggin. Th...
Tg(Cco.FGFR4del-Hsa.IGHG3del)

This construct encodes a secreted form of quail FGFR4. The extracellular portion of quail FGFR4 (ami...
Tg(sobpR651X)

This construct has an engineered mutation of the Xenopus sobp replicating a human mutation associate...
Tg(Hvu.wnt3)

Hydra vulgaris mRNA CDS for wnt3
Tg(Hsa.SHOX2)

Construct containing the human SHOX2 gene.
Tg(tbx5-EnR-GR)

A construct encoding the tbx5 transcription factor fused to the engrailed repressor domain and the h...
Tg(dnHsa.AKT1)

Dominant negative form of human AKT1. Has the following substitutions K179M T308A S473A. https://www...
Tg(HA-vangl2del1_84)

HA-tagged deletion mutants of Vangl2 Δ1-28 generated by site-directed mutagenesis or sub-cloning of ...
Tg(HA-vangl2del85_186)

HA-tagged deletion mutants of Vangl2 Δ29-62 generated by site-directed mutagenesis or sub-cloning of...
Tg(HA-vangl2del1_186)

HA-tagged deletion mutants of Vangl2 Δ1-62 generated by site-directed mutagenesis or sub-cloning of ...
Tg(HA-vangl2del894_1146)

HA-tagged deletion mutants of Vangl2 Δ298-382 generated by site-directed mutagenesis or sub-cloning ...
Tg(HA-vangl2T76>A,T78>A)

HA-tagged Vangl2 S/T>A mutations were generated by site-directed mutagenesis. This is a double muta...
Tg(Hsa.phf8)

Construct encoding the gene for the human PHF8 protein.
Tg(Eco.lacz)

Construct encoding the mRNA for the E. coli lacZ (beta-galactosidase) protein.
Tg(thradn)

The Xenopus DNTRα cDNA was generated by PCR by using pBluescript-TRαA,by deleting 42 base pairs (14 ...
Tg(Xla.pabpc1.LM161A)

Pabpc1.L{M161A} encodes a pabpc1.L mutant that is unable to bind eIf4g.
Tg(eGFP)

A template construct for generating eGFP mRNA, derived from pCS2+-eGFP.
Tg(Xtr.tubb2b:Hsa.ALPP)

Human ALPP ( aka placental alkaline phosphatase, PLAP) as a transgenic reporter. From PMID: 38439617...
Tg(Hsa.NEUROD2)

uman NEUROD2 mRNA generated from the Human ORFeome clone

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