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WISH with α-globin suggests that the blood distribution in mutant Xtr.smad4.1 tadpoles (on right, also known as whitehart or wha) is aberrant, with reduced globin staining pooled ventrally (black arrows) rather than distributed throughout the circulatory system as in wild-type tadpoles (left).
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hba1 (hemoglobin subunit alpha 1) gene expression in Xtr.smad4.1 mutant at tadpole stage, ( also known as whitehart or wha), asayyed bu WISH, ventral view, anterior left. This assays confirms phenotype as defects in blood formation with normal circulation.
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Xtr.smad4.1 mutant embryos. The white hart/wha phenotype includes no circulating blood cells, normal heartbeat, defect in blood formation, the dorsal fin appears 'frilly'.
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