XB-CLONE-12442459
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Clone Name: twist1-KL62
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Gene:
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Species: Xenopus laevis
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Description: EXRC Clone Number KL-62.
Xenopus laevis twist homolog 1.
To generate probe for in situ and mRNA for injection.
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Sequence:
Full length sequence: pXTwi560_FullLength
[+]
gNNNNNNNNNNNNNGCNGNNNTCTAGATGTGTGTGGCCTGAGCTGTAGTGGCATTGCTGTTAGTGAGATGCAGACATGGACCAGGCTCCCTCCATCCTCC
ACACGGAGAAGGCATAGCTGAGCCTCTCATGGGCCACATAACTGCAGCTGGCCATTTTGGAGTCCAGCTCGTCGCTCTGTAAGACCTGGCAGAGAAAGTC
GATGTATCTGGAGGCCAGTTTGAGCGTTTGGATTTTACTCAGTTTGTCCGAGGGGAGGGTGGGGATGATCTTTCTCAAGGACGAGAAGGCTTCGTTGAGG
GACTGAGTCCTCTGACGCTCCCTGACATTGGCCATGACCCTCTGACTCTGCAGCTCCTCAAAGGACTGGGGGCTACTGCCGGTGCTGCTCGCCTTCTTGT
TGCGCTTCACTGAGATCGGACTGTCGGGATCCTCGGGGCTTTTCCTAGCAGATCGCCTCTTTCTGCATCCCCTCTTGCTCTGCTGCTTGTCAAGCTCTTC
CTCGCTGTTACTCAGACTGTCTACTGGGGAGACTGGCGAGCTGGACTCTTCCTGCATCATTTTCTCCCGGCACAATAAGGAGAAGGTCCCGAATTCGATA
TCAAGCTTATCGATACCGTCGACCTCGAGGGGGGGCCCGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCAT
AGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGGAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTA
ACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGN
TTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGNTATCCACAGAATCAGGGGATAACGCANGAAAGAACATGTGAGCAAAANGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTC
CATAGGCTCCGCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCANAGGTGGCGAAACCCGACAGGACTNNNAAGATACCAGGCGTTTCCCCNGGA
AGCTCCNCNNNNNNTCTCNNTNGACCCNNCNNTACGGANNNCCNGTCGCTTCTCCTNGGNNNNNNGGNNNTTTNNNCNNAGCNNNANGCNNTANNNNNNN
CANTCGGNNNNAGGTCNNTNNNNNNNNCTGGGNNNGGNNNGNNNNNACCCCNNTNNNNCNACNCNNNNNNNTNNNGNANNNNNCNNNNNNNNCCNNNCNN
NNANNNNNNNNTNNNNNNNNNNNGNNNNNNNNCCNNNGG
Construction:
Insert:
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| Size: 560 bp | Source Tissue: | Stage: |
Vector:| Vector Details | Vector Map |
Name: pBluescript KS+
Type: phagemid
5' Restriction Site: null
3' Restriction Site: null |  |
Usage:
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RNA injection:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| sense |
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T3 |
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Whole mount in situ hybridization:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
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T7 |
EcoRI |
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Suppliers:
Search for clone at:
EXRC
