XB-CLONE-14573981
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Clone Name: fzd9-SPH85
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Gene:
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Species: Xenopus laevis
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Description: EXRC Clone Number SPH-85.
Xenopus laevis frizzled family receptor 9. Not full length lacks 5' end and initiation ATG.
To generate probe for in situ and Capped RNA.
Clone previously called pBSXfz9 - lambda1b.
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Sequence:
Full length sequence: pBSXfz9 - lambda1b_FullLength
[+]
gcgnnGCGGCGCTcTagaataGTGGaTCCCCCGGgCTGCAGGAATTCCGCAAACTAAAAATATCTTTTTATTTTAATAAAATAGTAAAGCATGAAAAATA
TCTGTATGTTACAAATATTTACAAGACAAGAGCAACACACAATACAAAGTTTCCCTTTGCAGAATCTAGTGCTTTTTTTtATCTTTTCATGGCTAGTATG
TGTTTCACAATAGAAAGAGTCAATAAGCCCTTTGAATTGATCCATCTCCCCCTTCTCTTTTTTTTTTTtAACCAAACACACCCACCGTGTATAACATTTG
GCTTGATTTCCATTAAAAAGCCTCAGAGAGCAAAGACTAAAGGGTTTGTGAGGGATAGACCCTTGAGCTCTTTTTTTGTTGCAGTTTATACAACAAGAGA
CGTGTTTCTCTTTTCAAGTCTGAGGGATTAAAGTGAAGATATTGCACTCTAGTTCCTACCGACAGCAGTCAGTCCTCAGCCATAGATTCTTCTGGCTATG
CCAAGAGCCAAGCAAACCCTACGCATGCTAAGAACTTAGGTGGGCACTGCCCTGTTGCAGCAATCCAGATCCTACTGAACTAACTACTATTTCCATGACT
GCTGTTTTAACATCTTTACCCAGTAACAGGTCAAGGGACACAATCACCAATACCGCAGGACCCGTGACGTCCAGCGATGTACACCAAATGTCATATCACT
AAAGGCAAAAGTAAAGATCTGCATTAGGGACCATTCGCTGAATGTAAGTAGGCAGGAAGAAGCCATTTTTTAGTTGCACAGTGCATTTCCTACTGTGTCA
ACACAGTAATGTACATTCTGTATTATAGGGGTGTGTGCAAAAGCAAAACACTTTCCCTGGCTAGTTCTAAnATGACCTGgAAAAtAGTATAAATATTTCT
TAAATACATGCTGTTTCAnATAACAGCTCTTTCTAATTTGGGCCTAGTGTCCCTTTAAGTTTCTCagaACTTCTAATCATTTACCCTCCCAGTCCCTTTC
CGTCCAGTTTCATGGAACTgntAAATTnncGATTtaaTTAGCATCTGACcannAATtAataGCCcGggAGgCc
Construction:
Insert:
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| Size: 3000 bp | Source Tissue: | Stage: |
Vector:| Vector Details | Vector Map |
Name: pBluescript KS+
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: null |  |
Usage:
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Whole mount in situ hybridization:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
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T7 |
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Linearize with Asp718. |
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Protein production:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| sense |
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T3 |
XbaI |
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Suppliers:
Search for clone at:
EXRC
