XB-CLONE-1818366
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Clone Name: tbx1-860
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Gene:
tbx1.L
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Species: Xenopus laevis
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Description: EXRC Clone Number 860.
Xenopus laevis T-box 1.
Image EST 7206683, Accession BC108602.
Full length.
To generate probe for in situ.
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Sequence:
Full length sequence: BC108602 
[+]
AGGGAACTGAGCGCACCTTCCCCATTCTGCTGCCCCACAGCCACTGCTCGGGATCCCCGCACCTTCCCAA
CCCCTACCCCACGGACAGAGTGGAGCAGC
AGCAGCACATTAACCCTTTGTTTTCTCCCCCCTCTTCCCTG
TATATTCCTGGGATGATCTCGGCCATCTCCAGCCCCTGGCTCACCCAGCTCTCCCATT
TTTGCGATGTTG
CAGCTTTCACGGCGAACAGCCTGAGCAGCTTGAACGCTTCGGGGGGCTACCACCTCTCCCCTTCCCCGGG
GGACCCGTACAGCCAG
CACGAACCCCACTACGAACCCTGCTCCGCCAGCCAACACTCGTACAGCTTCGGC
CATGCCTGCCCAGAGCCTGAGAGCGGAGCCTCGTCCTCTAGTTGC
GCCTCCTCCACCCCGGGGAGCGGCA
GTACAGGCAGCAGCAGCGGCAACAAGGCTCCGGTGAAGAAGAACCCCAAGGTGGCCAACATCACCGTGCA
GCT
GGAGATGAAAGCTCTGTGGGACGAGTTCAACCAGCTGGGCACTGAAATGATAGTCACCAAGGCTGGC
AGGCGTATGTTCCCCACCTTCCAGGTTAAGAT
TTTTGGGATGGATCCCATGGCCGATTACATGTTATTAA
TGGACTTTGTACCAGTGGACGATAAACGGTATCGGTATGCCTTTCACAGCTCCTCGTGGTT
AGTGGCAGG
CAAAGCAGACCCGGCCACACCCGGTAGAGTTCACTATCACCCGGATTCTCCAGCCAAGGGGGCTCAGTGG
ATGAAGCAGATCGTGTCCT
TTGACAAACTGAAACTGACAAATAACTTATTGGACGACAACGGGCACATCA
TCCTGAACTCCATGCACAGGTACCAGCCCCGCTTCCATGTGGTCTACG
TGGATCCGAGGAAGGACAGCGA
GAAGTACGCAGAGGAGAACTTTAAAACGTTCGTGTTCGAAGAGACGCGCTTTACGGCAGTGACGGCCTAT
CAGAAC
CACCGGATTACACAACTGAAAATTGCTAGCAATCCATTTGCCAAGGGATTCAGAGACTGTGACC
CAGAAGACTGGCCCAGAAATCATAGACCTGGATCC
CTCCCTCTTATGAATGCTTTTGCAAGGTCTAGGAA
TCCTGTCTCCTCCCCTCCACAGAATGGAAGTGATAAAGATGGAGATGGCCGAAGAGAATATGAA
AGAGAT
GCTAGTGTTACACCTCTGCATGGGGATGCAGCACATCAACAGCTTATGTCACGAGTACTCAGCCCGTCCC
TGCCTGTTCCTGGTGGACTTGT
ACCTCTCAGTACTGGCAGACCCAGCCCCCCACATGAACTGCGCCTAGA
CCCCCATTCTCAAGGCTCAGAACCACTGCACCACCATCCTTACAAGTACCC
AACCAGCTACGATCATTAC
CTAGGAGCAAAAACCCGACCGGCCCCCTACCCTTTGCCAAGTATCAGAGGACATGGCTACCATCATCACC
CTATGAACC
CAGCAGCAGCAAATATGTACTCGGGAGCTGGTGCACCAGGCGGTTATGAATACGGGCCAAG
ATAACATCTGCAGCAGGAGCCTATACTTATTGAATGAC
TATTTAATAAATATATAAGACAATGGATGTTT
CATGCATGATTTTAATTATTCAGTAGTGTATTGGATATTACATTACTGTTAAAATGAAAGCTGACCT
CTC
CCTAAAGTCACATGGGCAGCCCTGCAGCTTTATCAGTAATTTGTGAAAATCATCATGCAAACAAACTGGA
TCATCTAACAAATCCTGCTCGAGCT
GTTCCGATATTACATCAGCAGAAAGCAAGCAAAGGCACTTGAGTT
TGTCGTCTTTTTTCTTTTATTTGTTCAAATGCTAACTGGAAACAGAGGACAGAC
ACGTTGAAGAGGAGGT
GTCCAGGGACCTCTGTACATGCACATGTTTATTAAAGCTCCAAGTCTCCTTCTGCTTGTGGAGGAGATAG
TTCATCCCACCC
GACCTCCACAGATGCACTGTTCAATTACTAACTGACATCAGTCCACCCACCAAGGGCA
TTGACTTTTATGGGGCCTGGTGCACTAATAACGCTTGTAAG
AAGCCATATACTTTGTTTTGCTGTTGGTT
TTATTTTCAATTTCTTGAAAAAAAATGTTCAGTTGGCTCAGGTGCAGAGGAATTCTGAGGCGATATGTAG
TAAGCTACTTCTACCTGCCACTGTTGTTACCATAATCCACCCACTCATAAAAAATTTGTTCTTCCAAAGC
CATTTATCCATAACCCCTACTGACTGCA
TTAGTATGTAATGTCTCATCAAGAAATGTGTGTTTTGTGACT
CAAACAATTATAAATTTGCAGTGTATTTTCAAAATGTAAGTCTGTTTACCTCCCCTA
GATTTTTTTTTAT
CCAGTGACAAACTATAGGAAGGTGGGTGTACGGAAGTTTCCCATGATATTGCAGAACTATCATTTATCAA
AGGAATCCATCCAGG
TTGAAATATGGATCTCTAAGGGCAGGAGTTTCTAAAGTTACGTTTTTCTTTCTTC
TTATTTAACATGAAATTTTCATTTTGCACTTCATTAACACGCAG
TACACAGTGTACTGGAGCAAAATCAT
GGCGAATATGCTGTTTCTGGGGGGGGGGGGGCAAAGGGTGTAAACTGAAAAAATAAAACAGTAAACCAAT
TT
AAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CK798689 [+]ggaactgagcgcaccttccccattctgctgccccacagccactgctcgggatccccgcaccttcccaacccctaccccacggacagagtggagcagcagc
agcacattaaccctttgttttctcccccctcttccctgtatattcctgggatgatctcggccatctccagcccctggctcacccagctctcccatttttg
cgatgttgcagctttcacggcgaacagcctgagcagcttgaacgcttcggggggctaccacctctccccttccccgggggacccgtacagccagcacgaa
ccccactacgaaccctgctccgccagccaacactcgtacagcttcggccatgcctgcccagagcctgagagcggagcctcgtcctctagttgcgcctcct
ccaccccggggagcggcagtacaggcagcagcagcggcaacaaggctccggtgaagaagaaccccaaggtggccaacatcaccgtgcagctggagatgaa
agctctgtgggacgagttcaaccagctgggcactgaaatgatagtcaccaaggctggcaggcgtatgttccccaccttccaggttaagatttttgggatg
gatcccatggccgattacatgttattaatggactttgtaccagtggacgataaacggtatcggtatgcctttcacagctcctcgtggttagtggcaggca
aagcagacccggccacacccggtagagttcactatcacgcggattctccagccaggggggctcagtggatgaagcagatcgtgtcctttgacaaactgaa
actgacaaataaattattggaacaaaacgggccccttcatcctgaaatccctggccacgggatccgcccccgctttcctgttgggtctccggtggaattc
caagaaagg
Library:
Description: RNA obtained from 6 adult male testes. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1kb resulted in an average insert size of 1.15 kb. This primary, microquantity library is normalized to Cot5 (non-normalized primary library is NICHD_XGC_Te2) and was constructed by Express Genomics (Frederick, MD). Note: this is a Xenopus Gene Collection library.| Anatomy: testis | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pExpress-1
Type: plasmid
5' Restriction Site: EcoRV
3' Restriction Site: NotI |  |
Usage:
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Whole mount in situ hybridization:
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Expressed in branchial arches.
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
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T7 |
SacII |
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Suppliers:
Search for clone at:
EXRC
